利用感染性克隆cDNA研究脊髓灰质炎病毒。

Studying poliovirus with infectious cloned cDNA.

作者信息

Racaniello V R

出版信息

Rev Infect Dis. 1984 May-Jun;6 Suppl 2:S514-5. doi: 10.1093/clinids/6.supplement_2.s514.

DOI:10.1093/clinids/6.supplement_2.s514

Abstract

The application of molecular cloning to the study of poliovirus is discussed. Initially cDNA clones representing the entire genome of poliovirus type 1 (Mahoney) were prepared. These molecular clones were used for determining the complete nucleotide sequence of the viral genome. Subsequently a single cDNA clone representing the entire poliovirus genome was constructed. When this cDNA clone was transfected into cultured mammalian cells, infectious poliovirus was produced. The transfection-derived poliovirus was identical to wild-type virus by several criteria, including RNase T1-oligonucleotide fingerprint and 5' end nucleotide sequence. The availability of infectious cloned cDNA should make possible new approaches to the study of poliovirus.

摘要

讨论了分子克隆在脊髓灰质炎病毒研究中的应用。最初制备了代表1型脊髓灰质炎病毒（Mahoney株）全基因组的cDNA克隆。这些分子克隆被用于确定病毒基因组的完整核苷酸序列。随后构建了一个代表脊髓灰质炎病毒全基因组的单一cDNA克隆。当将这个cDNA克隆转染到培养的哺乳动物细胞中时，产生了有感染性的脊髓灰质炎病毒。通过包括核糖核酸酶T1 - 寡核苷酸指纹图谱和5'端核苷酸序列在内的几个标准，转染衍生的脊髓灰质炎病毒与野生型病毒相同。有感染性的克隆cDNA的可得性应该会为脊髓灰质炎病毒的研究带来新的方法。