由脊髓灰质炎病毒萨宾1型RNA合成的双链DNA拷贝的限制性图谱。
Restriction map of double-stranded DNA copy synthesized from poliovirus Sabin 1 RNA.
作者信息
Nomoto A, Toyoda H, Imura N, Noguchi S, Sekiya T
出版信息
J Biochem. 1982 May;91(5):1593-600. doi: 10.1093/oxfordjournals.jbchem.a133850.
Nearly full-sized double-stranded cDNA was prepared from virion RNA of poliovirus Sabin 1 (LSc, 2ab) strain using reverse transcriptase. The double-stranded cDNa was cleaved at four and two sites by the restriction endonucleases Bam HI and Hind III, respectively. Based on the cleavage patterns of double-stranded cDNA into segments of various lengths, each of which had the sequence corresponding to that of the 3'-end of the viral genome, the location of each restriction fragment was determined. The cDNA fragments were cloned with pBR322 as a vector and some of their nucleotide sequences were determined. The DNA sequence indicated that the restriction map obtained was consistent with the known arrangement of viral RNA fragments (1-3). Comparison of the nucleotide sequences of the cloned Hind III (460 bases) and Pst I (434 bases) fragments with those of the corresponding region of Mahoney type 1 genome (3) revealed 12 point-mutation sites.
使用逆转录酶从脊髓灰质炎病毒萨宾1型（LSc，2ab）毒株的病毒粒子RNA制备了几乎全长的双链cDNA。双链cDNA分别被限制性内切酶Bam HI和Hind III在四个和两个位点切割。基于双链cDNA切割成各种长度片段的模式，每个片段都具有与病毒基因组3'-末端相对应的序列，确定了每个限制性片段的位置。将cDNA片段用pBR322作为载体进行克隆，并测定了它们的一些核苷酸序列。DNA序列表明获得的限制性图谱与病毒RNA片段的已知排列一致（1-3）。将克隆的Hind III（460个碱基）和Pst I（434个碱基）片段的核苷酸序列与马奥尼1型基因组相应区域的序列进行比较（3），发现了12个点突变位点。
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