小鼠乳腺肿瘤病毒DNA甲基化：组织特异性变异

Mouse mammary tumor virus DNA methylation: tissue-specific variation.

作者信息

Breznik T, Traina-Dorge V, Gama-Sosa M, Gehrke C W, Ehrlich M, Medina D, Butel J S, Cohen J C

出版信息

Virology. 1984 Jul 15;136(1):69-77. doi: 10.1016/0042-6822(84)90248-4.

Abstract

Mouse mammary tumor virus-specific DNA sequences endogenous to the BALB/c mouse are shown to exhibit variable levels of methylation in a tissue-specific manner. In DNA from both lactating mammary gland and spleen, MMTV-specific sequences were hypomethylated at specific HpaII and HhaI sites. These variably methylated sites were found in the terminal repetitive sequences of the endogenous viral genomes. The specific hypomethylation of a HpaII site in Mtv-9 is associated with expression of a 1.6 kb transcript in the lactating mammary gland.

摘要

内源性BALB/c小鼠的小鼠乳腺肿瘤病毒特异性DNA序列以组织特异性方式呈现出不同水平的甲基化。在来自泌乳乳腺和脾脏的DNA中，MMTV特异性序列在特定的HpaII和HhaI位点发生低甲基化。这些可变甲基化位点存在于内源性病毒基因组的末端重复序列中。Mtv-9中一个HpaII位点的特异性低甲基化与泌乳乳腺中一个1.6 kb转录本的表达相关。