Marklund S L
Biochem J. 1984 May 15;220(1):269-72. doi: 10.1042/bj2200269.
A further characterization of human extracellular superoxide dismutase is reported. The study was especially aimed at the interaction with substances known to interfere with CuZn superoxide dismutase and other superoxide dismutases. Extracellular superoxide dismutase is efficiently inhibited by cyanide and is about 3 times more sensitive than is human CuZn superoxide dismutase. The sensitivity to azide is much lower, but still about 3 times higher than that of human CuZn superoxide dismutase. Extracellular superoxide dismutase is about as rapidly inactivated by hydrogen peroxide as is CuZn superoxide dismutase. The sensitivity to diethyldithiocarbamate is very high and more than an order of magnitude larger than that of CuZn superoxide dismutase. Sodium dodecyl sulphate, under conditions suggested as being suitable for distinguishing between the insensitive CuZn superoxide dismutase and the sensitive Mn superoxide dismutase, efficiently inactivated extracellular superoxide dismutase. No antigenic similarities between extracellular superoxide dismutase and CuZn superoxide dismutase could be demonstrated. Anti-(extracellular superoxide dismutase) did not bind CuZn superoxide dismutase, and anti-(CuZn superoxide dismutase) did not bind extracellular superoxide dismutase.
本文报道了对人细胞外超氧化物歧化酶的进一步特性研究。该研究特别针对与已知会干扰铜锌超氧化物歧化酶及其他超氧化物歧化酶的物质之间的相互作用。细胞外超氧化物歧化酶能被氰化物有效抑制，其敏感性约为人类铜锌超氧化物歧化酶的3倍。对叠氮化物的敏感性较低，但仍约为人类铜锌超氧化物歧化酶的3倍。细胞外超氧化物歧化酶被过氧化氢灭活的速度与铜锌超氧化物歧化酶大致相同。对二乙基二硫代氨基甲酸盐的敏感性非常高，比铜锌超氧化物歧化酶高一个多数量级。在被认为适合区分不敏感的铜锌超氧化物歧化酶和敏感的锰超氧化物歧化酶的条件下，十二烷基硫酸钠能有效灭活细胞外超氧化物歧化酶。未发现细胞外超氧化物歧化酶与铜锌超氧化物歧化酶之间存在抗原相似性。抗（细胞外超氧化物歧化酶）不结合铜锌超氧化物歧化酶，抗（铜锌超氧化物歧化酶）也不结合细胞外超氧化物歧化酶。
