Marklund S L
J Clin Invest. 1984 Oct;74(4):1398-403. doi: 10.1172/JCI111550.
Extracellular-superoxide dismutase is a tetrameric copper-containing glycoprotein that previously has been demonstrated to be the major superoxide dismutase of human extracellular fluids. The occurrence of this enzyme in various human tissues that were removed from two accidental death victims and in 19 different human cultured cell lines was determined. All investigated tissues were found to contain extracellular-superoxide dismutase. There was a larger variation between tissues in the concentration of this enzyme than in CuZn superoxide dismutase and Mn superoxide dismutase. No relation could be demonstrated between the content of extracellular-superoxide dismutase and the content of the other superoxide dismutase isoenzymes in the various tissues. In uterus there was more extracellular-superoxide dismutase than Mn superoxide dismutase, but in all other tissues the content of extracellular-superoxide dismutase was lower than the content of the other isoenzymes. The concentration of extracellular-superoxide dismutase was higher in all investigated human tissues than in human plasma. 19 human cultured cell lines were found to be devoid of or to contain very little extracellular-superoxide dismutase. Most tissues contained more extracellular-superoxide dismutase than did the investigated cell lines.
细胞外超氧化物歧化酶是一种含铜的四聚体糖蛋白，先前已被证明是人体细胞外液中的主要超氧化物歧化酶。测定了从两名意外死亡受害者身上取下的各种人体组织以及19种不同的人类培养细胞系中这种酶的存在情况。所有研究的组织都被发现含有细胞外超氧化物歧化酶。与铜锌超氧化物歧化酶和锰超氧化物歧化酶相比，这种酶在组织中的浓度变化更大。在各种组织中，细胞外超氧化物歧化酶的含量与其他超氧化物歧化酶同工酶的含量之间没有相关性。子宫中的细胞外超氧化物歧化酶比锰超氧化物歧化酶多，但在所有其他组织中，细胞外超氧化物歧化酶的含量低于其他同工酶的含量。在所有研究的人体组织中，细胞外超氧化物歧化酶的浓度都高于人体血浆中的浓度。发现19种人类培养细胞系不含或含有极少的细胞外超氧化物歧化酶。大多数组织中的细胞外超氧化物歧化酶比所研究的细胞系中的含量多。
