心房利钠因子（ANF）相关肽对肾上腺球状带细胞醛固酮分泌的影响：分子内二硫键的关键作用。

Effect of atrial natriuretic factor (ANF)-related peptides on aldosterone secretion by adrenal glomerulosa cells: critical role of the intramolecular disulphide bond.

作者信息

Chartier L, Schiffrin E, Thibault G

出版信息

Biochem Biophys Res Commun. 1984 Jul 18;122(1):171-4. doi: 10.1016/0006-291x(84)90455-8.

Abstract

We previously demonstrated that synthetic 48-73 atrial natriuretic factor (ANF) (previously called 8-33 ANF) blocked the response of rat adrenal glomerulosa cells to angiotensin II, ACTH and potassium. We have now investigated the effects of natural 43-73 ANF, oxidised synthetic 48-73 ANF and the natural 1-73 ANF on aldosterone output by rat glomerulosa cells. The natural 43-73 ANF and the natural 1-73 ANF were equipotent to 48-73 ANF in inhibiting the stimulation of aldosterone secretion produced by angiotensin II with an IC50 of 2 X 10(-9)M. Similar results were obtained with ACTH and potassium. After oxidation with performic acid, 48-73 ANF was completely devoid of activity on the response of aldosterone to angiotensin II, ACTH and potassium. We conclude that the intramolecular disulphide bond in 48-73 ANF is critical for maintaining the active conformation of ANF.

摘要

我们先前证明，合成的48 - 73心房利钠因子（ANF）（先前称为8 - 33 ANF）可阻断大鼠肾上腺球状带细胞对血管紧张素II、促肾上腺皮质激素（ACTH）和钾的反应。我们现在研究了天然的43 - 73 ANF、氧化的合成48 - 73 ANF和天然的1 - 73 ANF对大鼠球状带细胞醛固酮分泌的影响。天然的43 - 73 ANF和天然的1 - 73 ANF在抑制血管紧张素II引起的醛固酮分泌刺激方面与48 - 73 ANF等效，半数抑制浓度（IC50）为2×10⁻⁹M。用促肾上腺皮质激素（ACTH）和钾进行实验也得到了类似结果。经过甲酸氧化后，48 - 73 ANF对醛固酮对血管紧张素II、促肾上腺皮质激素（ACTH）和钾的反应完全失去活性。我们得出结论，48 - 73 ANF中的分子内二硫键对于维持ANF的活性构象至关重要。