猪肾微绒毛膜对α-人心房利钠肽的水解由内肽酶-24.11启动。
The hydrolysis of alpha-human atrial natriuretic peptide by pig kidney microvillar membranes is initiated by endopeptidase-24.11.
作者信息
Stephenson S L, Kenny A J
出版信息
Biochem J. 1987 Apr 1;243(1):183-7. doi: 10.1042/bj2430183.
Abstract
alpha-Human atrial natriuretic peptide, a 28-amino-acid-residue peptide, was rapidly hydrolysed by pig kidney microvillar membranes in vitro, with a t1/2 of 8 min, comparable with the rate observed with angiotensins II and III. The products of hydrolysis were analysed by h.p.l.c., the pattern obtained with membranes being similar to that with purified endopeptidase-24.11 (EC 3.4.24.11). No hydrolysis by peptidyl dipeptidase A (angiotensin I converting enzyme, EC 3.4.15.1) was observed. The contribution of the various microvillar membrane peptidases was assessed by including specific inhibitors. Phosphoramidon, an inhibitor of endopeptidase-24.11, caused 80-100% suppression of the products. Captopril and amastatin (inhibitors of peptidyl dipeptidase A and aminopeptidases respectively) had no significant effect. Hydrolysis at an undefined site within the disulphide-linked ring occurred rapidly, followed by hydrolysis at other sites, including the Ser25--Phe26 bond.
摘要
α-人心房利钠肽是一种由28个氨基酸残基组成的肽，在体外可被猪肾微绒毛膜迅速水解，半衰期为8分钟，这与血管紧张素II和III的水解速率相当。水解产物通过高效液相色谱进行分析，用膜得到的图谱与用纯化的内肽酶-24.11（EC 3.4.24.11）得到的图谱相似。未观察到肽基二肽酶A（血管紧张素I转换酶，EC 3.4.15.1）的水解作用。通过加入特异性抑制剂来评估各种微绒毛膜肽酶的作用。磷酰胺素是内肽酶-24.11的抑制剂，可导致80-100%的产物抑制。卡托普利和抑氨肽酶素（分别为肽基二肽酶A和氨肽酶的抑制剂）没有显著作用。在二硫键连接环内的一个未确定位点的水解迅速发生，随后在其他位点水解，包括Ser25-Phe26键。
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