Minnich M, Kueppers F, James H
Comp Biochem Physiol B. 1984;78(2):413-9. doi: 10.1016/0305-0491(84)90051-8.
Alpha-1-antitrypsin (alpha-1-protease inhibitor) was isolated from mouse serum by a series of electrophoretic and chromatographic steps. We found it to be a glycoprotein of a mass ratio of 57.7 Kd. The extinction coefficient was E1%1cm,280=4.74. It inhibits bovine trypsin, human granulocytic and porcine pancreatic elastase. Its concentration in serum differs between inbred strains. Of those tested the concentration in C57BL/6J males was lowest with 5.58 +/- 0.71 mg/ml (females: 3.02 +/- 0.39) and that in DBA/2J was highest: 8.5 +/- 0.87 mg/ml (females: 4.09 +/- 0.51). The concentration of alpha-1-antitrypsin in male serum was almost twice as high as that in females of all strains tested.
通过一系列电泳和色谱步骤从小鼠血清中分离出α-1-抗胰蛋白酶（α-1-蛋白酶抑制剂）。我们发现它是一种质量比为57.7 Kd的糖蛋白。消光系数为E1%1cm,280 = 4.74。它抑制牛胰蛋白酶、人粒细胞弹性蛋白酶和猪胰弹性蛋白酶。其在血清中的浓度在近交系之间有所不同。在测试的那些品系中，C57BL/6J雄性的浓度最低，为5.58±0.71 mg/ml（雌性：3.02±0.39），而DBA/2J中的浓度最高：8.5±0.87 mg/ml（雌性：4.09±0.51）。在所有测试品系中，雄性血清中α-1-抗胰蛋白酶的浓度几乎是雌性的两倍。
