Layton G T, Stanworth D R
J Immunol Methods. 1984 Oct 26;73(2):347-56. doi: 10.1016/0022-1759(84)90410-1.
Enzyme-linked immunosorbent assays, capable of detecting nanogram quantities of IgG4 to 3 common food allergens, have been developed with use of a monoclonal anti-human IgG4. Calibrated positive human sera were used to construct standard curves. The allergens employed, major protein constituents of egg (ovalbumin), milk (beta-lactoglobulin) and-wheat (gluten), were highly effective in coating microtitre plate wells; and the assays proved to be sensitive, reproducible and specific, in terms of both allergen and IgG subclass. On testing sera from a group of non-allergic individuals a high proportion were found to contain IgG4 antibodies to the 3 allergens. A pool of 60 sera obtained from blood donors also contained IgG4 antibodies to all 3 allergens. The results illustrate that IgG4 antibodies against common food constituents are frequently encountered in sera from normal individuals; and that this needs to be taken into consideration in assessing the possible role of such antibodies in immediate hypersensitivity reactions.
利用单克隆抗人IgG4，已开发出能够检测纳克级量的针对3种常见食物过敏原的IgG4的酶联免疫吸附测定法。使用校准的阳性人血清构建标准曲线。所使用的过敏原，即鸡蛋（卵清蛋白）、牛奶（β-乳球蛋白）和小麦（麸质）的主要蛋白质成分，在包被微量滴定板孔方面非常有效；并且这些测定法在过敏原和IgG亚类方面均被证明是灵敏、可重复且特异的。在检测一组非过敏个体的血清时，发现很大一部分人含有针对这3种过敏原的IgG4抗体。从献血者获得的60份血清混合样本中也含有针对所有3种过敏原的IgG4抗体。结果表明，正常个体的血清中经常会遇到针对常见食物成分的IgG4抗体；并且在评估此类抗体在速发型超敏反应中可能发挥的作用时需要考虑到这一点。
