Persson U, Johansson S G
Int Arch Allergy Appl Immunol. 1984;75(4):337-44. doi: 10.1159/000233644.
The proliferative response to concanavalin A (Con A) in human peripheral lymphocytes is dependent on the presence of adherent cells. OKT 8-positive cells respond less well to Con A than OKT 4-positive cells, especially to high concentrations of the activator. The response in both subpopulations of cells is dependent on the number of adherent cells added, the dose of the mitogen used, the day of testing as well as the presence of lymphocyte-produced factors. The data presented in the paper clearly show the importance of caution in evaluation of results obtained by in vitro-induced mitogen activation due to the number of known and unknown variables that affect the magnitude of the response.
人外周血淋巴细胞对刀豆蛋白A（Con A）的增殖反应取决于贴壁细胞的存在。OKT 8阳性细胞对Con A的反应不如OKT 4阳性细胞，尤其是对高浓度激活剂的反应。两个细胞亚群中的反应均取决于添加的贴壁细胞数量、所用丝裂原的剂量、检测的日期以及淋巴细胞产生的因子的存在。本文给出的数据清楚地表明，由于影响反应强度的已知和未知变量众多，在评估体外诱导的丝裂原激活所获得的结果时必须谨慎。
