Holán V, Nakamura S, Minowada J
Fujisaki Cell Center, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
Immunology. 1992 Jan;75(1):176-81.
Highly purified natural human interferon-alpha (IFN-alpha) inhibited in a dose-dependent manner the proliferation of human peripheral blood lymphocytes (PBL) stimulated with T-cell mitogen concanavalin A (Con A) or with interleukin-2 (IL-2). Contrary to this inhibitory effect, IFN-alpha at the same concentrations significantly increased proliferation of PBL stimulated with B-cell mitogen bacterial lipopolysaccharide (LPS) or with IL-3, and even spontaneous proliferation of PBL was enhanced by IFN-alpha. Proliferation of Con A-stimulated PBL depleted of CD8+ cells was sensitive to the inhibitory action of IFN-alpha, while proliferation of the Con A-stimulated CD4+ cell-depleted PBL was not affected by IFN-alpha. The inhibitory effect of IFN-alpha on PBL proliferation was due to neither inhibition of IL-2 receptor (IL-2R) expression, activation of suppressor cells, nor inhibition of lymphokine production. Rather, IFN-alpha augmented production of IL-1 and IL-2 by PBL. These results show that the suppressive effect of natural IFN-alpha on Con A-induced proliferation of PBL is due to a direct growth-inhibitory effect on CD4+ T cells, and that IFN-alpha simultaneously augments production of lymphokines. This could in turn lead to the increased proliferation of IFN-alpha-resistant cell populations.
高度纯化的天然人α干扰素（IFN-α）以剂量依赖的方式抑制了用T细胞丝裂原刀豆球蛋白A（Con A）或白细胞介素-2（IL-2）刺激的人外周血淋巴细胞（PBL）的增殖。与这种抑制作用相反，相同浓度的IFN-α显著增加了用B细胞丝裂原细菌脂多糖（LPS）或IL-3刺激的PBL的增殖，甚至IFN-α增强了PBL的自发增殖。去除CD8+细胞的Con A刺激的PBL的增殖对IFN-α的抑制作用敏感，而去除Con A刺激的CD4+细胞的PBL的增殖不受IFN-α的影响。IFN-α对PBL增殖的抑制作用既不是由于抑制白细胞介素-2受体（IL-2R）的表达、抑制细胞的激活，也不是由于抑制淋巴因子的产生。相反，IFN-α增强了PBL产生IL-1和IL-2的能力。这些结果表明，天然IFN-α对Con A诱导的PBL增殖的抑制作用是由于对CD4+T细胞的直接生长抑制作用，并且IFN-α同时增强了淋巴因子的产生。这反过来可能导致对IFN-α耐药的细胞群体的增殖增加。
