艾滋病患者淋巴细胞在体外有丝分裂原刺激下的T细胞表面抗原表达。

T cell surface antigen expression on lymphocytes of patients with AIDS during in vitro mitogen stimulation.

作者信息

Munn C G, Reuben J M, Hersh E M, Mansell P W, Newell G R

出版信息

Cancer Immunol Immunother. 1984;18(3):141-8. doi: 10.1007/BF00205502.

DOI:10.1007/BF00205502

PMID:6334551

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11039248/

Abstract

Surface marker expression on peripheral blood mononuclear cells (PBMC) was evaluated daily in PHA- and PWM-stimulated cultures of eight AIDS patients and eight normals. Before culture, the patients' cells showed the characteristic decrease in OKT 4+ cells (normals 40.4%, patients 22.3%; P less than 0.001), increase in OKT 8+ cells (normals 27.6%, AIDS 38.4%; P = 0.002), increase in OKT 10+ cells (normals 15.5%, AIDS 42.8%; P = 0.002), and increase in HLA-DR+ cells (normals 11.4%, AIDS 28.7%; P = 0.01). The percentage of OKT 11+ cells remained unchanged, while the percentage of OKT 3+ cells dropped over the first 2 days in PHA but not in PWM cultures of both groups (PHA: normals 69.8% to 35.1%; P = 0.001, AIDS 56.5 to 38.5%; P = 0.001, PWM: normals 62.8%-65.9%, AIDS 66.8% to 63.9%), and recovered in both groups by day 5. In PWM cultures OKT 3+ cells increased significantly in normals but not in AIDS (normals 62.6%-77.7%; P = 0.04, AIDS 61.8 to 48.7%). OKT 4 expression decreased in normal PHA cultures after 1 day (38.9% to 29.6%; P = 0.05) and then recovered by day 5. Its expression increased in AIDS PHA cultures by day 5 (18.0%-41.1%; P less than 0.001). The final percentage of OKT 4+ cells in AIDS cultures was within the normal range (35.0%-49.0%). OKT 8 expression increased in both study groups after PHA stimulation (normals 29.5%-50.4%; P = 0.002, AIDS 37.4%-50.7%; P = 0.02) and in normals but not AIDS after PWM stimulation (normals 28.9%-35.5%; P = 0.004, AIDS 38.5%-35.6%). Because of the relative changes in expression of OKT 4 and OKT 8, the 4/8 ratio declined in the normal PHA cultures (1.89 to 1.03; P = 0.1) and increased in the AIDS cultures (0.68-1.18; P = 0.09). Also, the sum of OKT 4+ and OKT 8+ cells in PHA cultures increased from 68% to 94% whilst expression of OKT 11 remained unchanged, indicating co-expression of these antigens on individual cells. Both PHA-and PWM-stimulated normal cells showed an increase in OKT 10 (PHA 16.0%-53.4%; P = 0.01, PWM 16.1%-33.9%; P = 0.03) and HLA-DR (PHA 8.6%-27.3%; P = 0.03, PWM 12.5%-26.6%; P = 0.07). In AIDS PHA cultures this did not change, and in their PWM cultures OKT 10 expression declined (44.8 to 23.0%; P = 0.05).(ABSTRACT TRUNCATED AT 400 WORDS)

摘要

对8名艾滋病患者和8名正常人的外周血单个核细胞（PBMC）进行PHA和PWM刺激培养，并每日评估其表面标志物表达情况。培养前，患者细胞显示OKT 4 +细胞特征性减少（正常人40.4%，患者22.3%；P<0.001），OKT 8 +细胞增加（正常人27.6%，艾滋病患者38.4%；P = 0.002），OKT 10 +细胞增加（正常人15.5%，艾滋病患者42.8%；P = 0.002），HLA - DR +细胞增加（正常人11.4%，艾滋病患者28.7%；P = 0.01）。OKT 11 +细胞百分比保持不变，而两组PHA培养物中OKT 3 +细胞百分比在最初2天下降（PHA：正常人从69.8%降至35.1%；P = 0.001，艾滋病患者从56.5%降至38.5%；P = 0.001，PWM：正常人62.8% - 65.9%，艾滋病患者66.8%至63.9%），两组均在第5天恢复。在PWM培养物中，正常人OKT 3 +细胞显著增加，而艾滋病患者未增加（正常人62.6% - 77.7%；P = 0.04，艾滋病患者61.8%至48.7%）。正常PHA培养物中OKT 4表达在1天后下降（38.9%至29.6%；P = 0.05），然后在第5天恢复。艾滋病PHA培养物中其表达在第5天增加（18.0% - 41.1%；P<0.001）。艾滋病培养物中OKT 4 +细胞的最终百分比在正常范围内（35.0% - 49.0%）。PHA刺激后，两个研究组中OKT 8表达均增加（正常人29.5% - 50.4%；P = 0.002，艾滋病患者37.4% - 50.7%；P = 0.02），PWM刺激后，正常人增加而艾滋病患者未增加（正常人28.9% - 35.5%；P = 0.004，艾滋病患者从38.5%降至35.6%）。由于OKT 4和OKT 8表达的相对变化，正常PHA培养物中4/8比值下降（从1.89降至1.03；P = 0.1），艾滋病培养物中增加（从0.68升至1.18；P = 0.09）。此外，PHA培养物中OKT 4 +和OKT 8 +细胞的总和从68%增加到94%，而OKT 11表达保持不变，表明这些抗原在单个细胞上共表达。PHA和PWM刺激的正常细胞中OKT 10（PHA从16.0%升至53.4%；P = 0.01，PWM从16.1%升至33.9%；P = 0.03）和HLA - DR（PHA从8.6%升至27.3%；P = 0.03，PWM从12.5%升至26.6%；P = 0.07）均增加。在艾滋病PHA培养物中这没有变化，在其PWM培养物中OKT 10表达下降（从44.8%降至23.0%；P = 0.05）。（摘要截短至400字）