Townson S, Bianco A E, Doenhoff M J, Muller R
Tropenmed Parasitol. 1984 Dec;35(4):202-8.
The model of Onchocerca lienalis microfilariae injected into inbred CBA/HT6T6 mice has been examined for its value to study immunity to the skin-dwelling microfilariae in onchocerciasis. Mice injected with living microfilariae during primary or secondary infections exhibited a high level of resistance to challenge relative to normal controls (91-98% reduction in recoveries). The survival of microfilariae during a primary infection was significantly prolonged in T-cell deprived animals compared with immunologically intact mice. Serum and spleen cells transferred from donors 90 days after infection conferred significant protection in syngeneic recipients to challenge with microfilariae (59% reduction in recoveries compared with controls). Boostered injections with freeze-killed or fragmented microfilariae reduced parasite recoveries after challenge by 39-78%: none of 5 adjuvant preparations enhanced the protective effect. Mice exposed to living infective larvae or adult males of O. lienalis also exhibited lowered recoveries of microfilariae following a challenge infection. It is concluded that the mouse model offers potential for immunological studies on the microfilariae in onchocerciasis, which have hitherto been limited because of the lack of suitable laboratory hosts.
已对将链尾盘尾丝虫微丝蚴注射到近交系CBA/HT6T6小鼠体内的模型进行了研究，以评估其在研究盘尾丝虫病中针对皮肤寄生微丝蚴的免疫方面的价值。在初次或二次感染期间注射活微丝蚴的小鼠相对于正常对照表现出对攻击的高度抗性（回收率降低91-98%）。与免疫健全的小鼠相比，T细胞缺乏的动物在初次感染期间微丝蚴的存活时间显著延长。感染90天后从供体转移的血清和脾细胞给予同基因受体显著的保护，使其免受微丝蚴攻击（与对照相比回收率降低59%）。用冻融或破碎的微丝蚴进行加强注射可使攻击后寄生虫的回收率降低39-78%：5种佐剂制剂均未增强保护作用。暴露于链尾盘尾丝虫活感染性幼虫或成年雄虫的小鼠在攻击感染后微丝蚴的回收率也降低。得出的结论是，该小鼠模型为盘尾丝虫病中微丝蚴的免疫学研究提供了潜力，此前由于缺乏合适的实验室宿主，此类研究一直受到限制。
