Townson S, Bianco A E
J Helminthol. 1982 Dec;56(4):297-303. doi: 10.1017/s0022149x00034684.
Three Jersey bull calves were immunized by subcutaneous injections of sonicated Onchocerca lienalis microfilariae suspended in phosphate buffered saline, and three control animals were injected with medium only. All calves received booster injections 27 days later, and were challenged with live microfilariae 44 days after the booster. Following challenge, only the immunized animals developed an elevated level of circulating eosinophils. When necropsied ten days after challenge there was a 97% reduction in recoveries of microfilariae from immunized animals compared to challenge controls. In human onchocerciasis it is the microfilariae which are the principal cause of pathology, and we believe that studies on O. lienalis in both the natural bovine host and in inbred rodents provide a promising model to investigate immunity to Onchocerca microfilariae.
三只泽西公牛犊通过皮下注射悬浮于磷酸盐缓冲盐溶液中的经超声处理的盘尾丝虫微丝蚴进行免疫，三只对照动物仅注射培养基。所有牛犊在27天后接受加强注射，并在加强注射后44天用活的微丝蚴进行攻击。攻击后，只有免疫动物的循环嗜酸性粒细胞水平升高。在攻击后十天进行尸检时，与攻击对照组相比，免疫动物体内微丝蚴的回收率降低了97%。在人类盘尾丝虫病中，微丝蚴是病理变化的主要原因，我们认为对自然牛宿主和近交啮齿动物体内的盘尾丝虫进行研究，为研究针对盘尾丝虫微丝蚴的免疫提供了一个有前景的模型。
