Meyers-Elliott R H, Jacobs D R, Gammon R A
J Neuroimmunol. 1983 Feb;4(1):25-34. doi: 10.1016/0165-5728(83)90061-9.
The presence and localization of autoantibodies was determined in strain 13 guinea pigs with experimental retinal autoimmunity (ERA) induced by immunization with rhodopsin and rod outer segments (ROS). Sera were obtained from rhodopsin-immunized and from ROS-immunized guinea pigs before, during, and after onset of clinical uveitis. Autoantibodies were detected by indirect immunofluorescent staining of autogenic retinas as well as normal guinea pig retinas. Sera from animals with clinical disease showed specific labeling of the photoreceptor cell layer of the retina. The rhodopsin autoantibody showed a more defined specificity than the ROS autoantibody staining, only the retinal photoreceptors and retinal pigment epithelium. Specific fluorescence was localized only in the retina, and not in any other ocular or nonocular tissues. Neither the rhodopsin nor the ROS antibodies stained the uvea. Sera from animals taken before the onset of clinical disease did not demonstrate the presence of retinal-binding autoantibodies. These findings suggest that photoreceptor-binding autoantibodies appear in the sera of animals immunized with rhodopsin and with ROS, but only in animals with clinical disease. However, these antibodies probably are not the primary cause of pathology, since previous passive transfer experiments (data not shown here) could not be achieved with anti-ROS or with anti-rhodopsin antibodies. These autoantibodies could occur secondarily as a response to the bovine antigens which cross-reacted with the autologous guinea pig antigens. Subsequently these antibodies could be of primary importance in further tissue alteration and destruction.
通过用视紫红质和视杆外段（ROS）免疫诱导实验性视网膜自身免疫（ERA）的13号品系豚鼠，来确定自身抗体的存在及其定位。在临床葡萄膜炎发作之前、期间和之后，从用视紫红质免疫和用ROS免疫的豚鼠中获取血清。通过对自体视网膜以及正常豚鼠视网膜进行间接免疫荧光染色来检测自身抗体。患有临床疾病的动物血清显示视网膜感光细胞层有特异性标记。视紫红质自身抗体显示出比ROS自身抗体染色更明确的特异性，仅标记视网膜感光细胞和视网膜色素上皮。特异性荧光仅定位于视网膜，而不在任何其他眼组织或非眼组织中。视紫红质抗体和ROS抗体均未对葡萄膜进行染色。临床疾病发作前采集的动物血清未显示存在视网膜结合自身抗体。这些发现表明，在用视紫红质和ROS免疫的动物血清中出现了感光细胞结合自身抗体，但仅在患有临床疾病的动物中出现。然而，这些抗体可能不是病理的主要原因，因为先前的被动转移实验（此处未显示数据）无法用抗ROS抗体或抗视紫红质抗体实现。这些自身抗体可能作为对与自体豚鼠抗原发生交叉反应的牛抗原的反应而继发出现。随后，这些抗体可能在进一步的组织改变和破坏中起主要作用。
