Introna M, Allavena P, Biondi A, Colombo N, Villa A, Mantovani A
J Natl Cancer Inst. 1983 Jan;70(1):21-6.
Tumor-associated lymphocytes (TAL) were isolated from 17 ascites and 7 solid ovarian carcinomas. TAL had defective natural killer (NK) activity against K562. Large granular lymphocytes, the morphologically identified effectors of NK activity, were poorly represented in TAL from ovarian carcinomas as compared to peripheral blood lymphocytes from the same patients or from normal donors. Similar results were obtained when effectors of NK activity were identified with an anti-NK (HNK-1) monoclonal antibody. When four TAL preparations were separated on discontinuous Percoll gradients, they were able to be enriched for NK activity and large granular lymphocyte morphology in the lower density fractions as observed with blood. These observations suggested that a low concentration of the relevant effector cells was the major factor determining the defective NK cytotoxicity of lymphoid cells associated with these human neoplasms.
从17例腹水型和7例实体型卵巢癌中分离出肿瘤相关淋巴细胞（TAL）。TAL对K562的自然杀伤（NK）活性存在缺陷。形态学上确定的NK活性效应细胞——大颗粒淋巴细胞，与同一患者或正常供体的外周血淋巴细胞相比，在卵巢癌的TAL中所占比例较低。当用抗NK（HNK-1）单克隆抗体鉴定NK活性效应细胞时，也得到了类似结果。当四种TAL制剂在不连续的Percoll梯度上分离时，与血液情况一样，它们能够在较低密度级分中富集NK活性和大颗粒淋巴细胞形态。这些观察结果表明，相关效应细胞浓度低是决定与这些人类肿瘤相关的淋巴细胞NK细胞毒性缺陷的主要因素。
