Soloway M S, Nissenkorn I, McCallum L
Urology. 1983 Feb;21(2):159-61. doi: 10.1016/0090-4295(83)90013-4.
To determine whether or not transitional cell carcinoma cells will preferentially implant and grow on an altered urothelial surface, cauterization and instillation of N-methyl-N-nitrosourea (MNU) were used to alter the murine bladder urothelium. Transplantable tumor cells (2.09 X 10(6) ) were placed into the bladders of 53 mice. Tumor cell implantation occurred in only 6 per cent of the mice with a normal bladder, whereas tumors were present in 28 per cent of mice pretreated with intravesical MNU and in 67 per cent of mice which had a portion of the bladder cauterized prior to insertion of tumor cells (p less than 0.005). This study not only establishes the optimal technique for implantation in this experimental model, but also suggests that seeding may be a contributory factor in the high recurrence rate following endoscopic treatment of bladder tumors in man.
为了确定移行细胞癌细胞是否会优先种植并生长在改变的尿路上皮表面，采用烧灼和灌注N-甲基-N-亚硝基脲（MNU）来改变小鼠膀胱尿路上皮。将可移植的肿瘤细胞（2.09×10⁶）植入53只小鼠的膀胱。正常膀胱的小鼠中只有6%发生肿瘤细胞植入，而膀胱内灌注MNU预处理的小鼠中有28%出现肿瘤，在植入肿瘤细胞前部分膀胱被烧灼的小鼠中有67%出现肿瘤（p<0.005）。本研究不仅确立了该实验模型中植入的最佳技术，还提示种植可能是人类膀胱肿瘤内镜治疗后高复发率的一个促成因素。
