Tager J M, Wanders R J, Groen A K, Kunz W, Bohnensack R, Küster U, Letko G, Böhme G, Duszynski J, Wojtczak L
FEBS Lett. 1983 Jan 10;151(1):1-9. doi: 10.1016/0014-5793(83)80330-5.
The control theory of Kacser and Burns [in: Rate Control of Biological Processes (Davies, D.D. ed) pp. 65-104, Cambridge University Press, London, 1973] and Heinrich and Rapoport [Eur. J. Biochem. (1974) 42, 97-105] has been used to quantify the amount of control exerted by different steps on mitochondrial oxidative phosphorylation in rat-liver mitochondria. Inhibitors were used to manipulate the amount of active enzyme. The control strength of the adenine nucleotide translocator was measured by carrying out titrations with carboxyatractyloside. In state 4, the control strength of the translocator was found to be zero. As the rate of respiration was increased by adding hexokinase, the control strength of the translocator increased to a maximum value of approximately 30% at approximately 80% of state 3 respiration. In state 3, control of respiration is distributed between a number of steps, including the adenine nucleotide translocator, the dicarboxylate carrier and cytochrome c oxidase. The measured values for the distribution of control agree very well with those calculated with the aid of a model for mitochondrial oxidative phosphorylation developed by Bohnensack et al. [Biochim. Biophys. Acta (1982) 680, 271-280].
卡塞尔和伯恩斯的控制理论[载于《生物过程的速率控制》（戴维斯，D.D. 编）第65 - 104页，剑桥大学出版社，伦敦，1973年]以及海因里希和拉波波特的理论[《欧洲生物化学杂志》（1974年）42卷，97 - 105页]已被用于量化不同步骤对大鼠肝脏线粒体氧化磷酸化所施加的控制量。使用抑制剂来调控活性酶的量。通过用羧基苍术苷进行滴定来测量腺嘌呤核苷酸转位酶的控制强度。在状态4下，发现转位酶的控制强度为零。随着通过添加己糖激酶来增加呼吸速率，转位酶的控制强度在大约状态3呼吸的80%时增加到最大值约30%。在状态3下，呼吸控制分布在多个步骤之间，包括腺嘌呤核苷酸转位酶、二羧酸载体和细胞色素c氧化酶。控制分布的测量值与借助博嫩萨克等人[《生物化学与生物物理学报》（1982年）680卷，271 - 280页]开发的线粒体氧化磷酸化模型计算得到的值非常吻合。
