Kettenmann H, Orkand R K, Schachner M
J Neurosci. 1983 Mar;3(3):506-16. doi: 10.1523/JNEUROSCI.03-03-00506.1983.
Cell coupling can be demonstrated among groups of oligodendrocytes, astrocytes, and fibroblasts or fibroblast-like cells, but not for dorsal root ganglion neurons, Schwann cells, or macrophages in mouse or rat cell cultures. Neurons were identified by their ability to generate action potentials. Non-neuronal cells were recognized immunologically by their ability to react with a variety of rhodamine-labeled cell type-specific antibodies. Intracellular injection of the gap junction permeable fluorescent dye Lucifer Yellow and ionic current were used to establish the presence of coupling among these identified cells. Coupling under the culture conditions used in this study does not represent a random membrane interaction between closely apposed cells but rather a form of communication among restricted populations.
在小鼠或大鼠细胞培养物中，少突胶质细胞、星形胶质细胞、成纤维细胞或成纤维细胞样细胞群体之间可表现出细胞耦联，但背根神经节神经元、雪旺细胞或巨噬细胞则不会出现这种情况。神经元通过其产生动作电位的能力来识别。非神经元细胞通过其与多种罗丹明标记的细胞类型特异性抗体发生反应的能力进行免疫识别。通过细胞内注射可透过缝隙连接的荧光染料鲁米诺黄和离子电流来确定这些已识别细胞之间是否存在耦联。本研究中所用培养条件下的耦联并非紧密相邻细胞之间的随机膜相互作用，而是特定细胞群体之间的一种通讯形式。
