Levin D E, Yamasaki E, Ames B N
Mutat Res. 1982 Jun;94(2):315-30. doi: 10.1016/0027-5107(82)90294-9.
We have developed a new Salmonella tester strain, TA97, for use in the Salmonella/microsome mutagenicity test. DNA sequencing has shown that this strain contains an added cytosine, resulting in a run of six cytosines at the mutated site in the histidine D gene. Its mutagenic specificity is similar to that of the frameshift mutagen tester strain, TA1537, which also contains an added cytosine in a run of cytosines and is currently among the five standard tester strains used for general mutagen screening. We assessed the mutagenic potency of 21 frameshift mutagens for TA1537 and TA97. TA97 was considerably more sensitive than TA1537 to reversion by these frameshift mutagens. In addition, one agent, PR toxin (from Penicillium roqueforti), which was not detected by any of the previously existing standard tester strains, did revert TA97; and two substituted aryl-alkyl triazenes, which had not been reported previously to be frameshift mutagens, were mutagenic in this new tester strain. We suggest that TA1537 be replaced by TA97 for general screening of mutagenicity.
我们开发了一种新的沙门氏菌测试菌株TA97，用于沙门氏菌/微粒体致突变性试验。DNA测序表明，该菌株含有一个额外的胞嘧啶，导致组氨酸D基因的突变位点出现六个胞嘧啶的连续排列。其诱变特异性与移码诱变测试菌株TA1537相似，TA1537在胞嘧啶连续排列中也含有一个额外的胞嘧啶，目前是用于一般诱变筛选的五个标准测试菌株之一。我们评估了21种移码诱变剂对TA1537和TA97的诱变效力。TA97对这些移码诱变剂的回复突变比TA1537敏感得多。此外，一种试剂PR毒素（来自罗克福青霉），以前任何现有的标准测试菌株都未检测到它能使TA97回复突变；还有两种取代芳基烷基三氮烯，以前未报道它们是移码诱变剂，但在这种新的测试菌株中具有致突变性。我们建议用TA97取代TA1537进行一般的诱变性筛选。
