Michaels L, Wells M, Frohlich A
Clin Otolaryngol Allied Sci. 1983 Apr;8(2):77-85. doi: 10.1111/j.1365-2273.1983.tb01410.x.
A new technique for the study of the temporal bone at autopsy is presented in which thin slices of the fixed bone are prepared on a special cutting machine which preserves the configuration of the delicate anatomical structures. Selected areas are then processed for light microscopy. There is thus opportunity for gross examination, only a short period for immersion in acid for decalcification if required, and special histological procedures may also be carried out. The method is quick and technically easier than serial sectioning and storage of the smaller numbers of sections from each case is less of a problem. Temporal bones which have been perfused through the perilymph within 3 h of death may also be cut in the same way in order to allow removal of samples of membranous labyrinth for surface preparation or electron microscopy, avoiding the need for drilling to reach the membranous labyrinth, and being quicker and less damaging.
本文介绍了一种用于尸检时颞骨研究的新技术。在这种技术中,将固定好的骨头切成薄片,使用一种特殊的切割机来制备,该切割机能够保留精细解剖结构的形态。然后对选定区域进行光学显微镜检查。这样就有机会进行大体检查,如果需要,只需在酸中浸泡较短时间进行脱钙,还可以进行特殊的组织学程序。该方法快速,并且在技术上比连续切片更容易,而且每个病例保存较少数量的切片也不成问题。对于在死亡后3小时内通过外淋巴灌注的颞骨,也可以以同样的方式进行切割,以便取出膜迷路样本进行表面制备或电子显微镜检查,避免了钻孔到达膜迷路的需要,而且更快且损伤更小。