Scheld W M, Calderone R A, Brodeur J P, Sande M A
Infect Immun. 1983 Jun;40(3):950-5. doi: 10.1128/iai.40.3.950-955.1983.
The influence of preformed antibody on the induction of experimental Candida albicans endocarditis was investigated by both in vitro and in vivo techniques. Preincubation of C. albicans with immune serum (raised in rabbits by intravenous injection of Formalin-killed yeast cells) decreased adhesion to the constituents of nonbacterial thrombotic endocarditis, e.g., fibrin plus platelets, in vitro. Two different methods, with radiolabeled or viable yeast cells, were confirmatory and demonstrated decreased adhesion of immune serum-treated C. albicans cells to 0 to 7.8% of control values (P less than 0.001). These results correlated with protection from the development of C. albicans endocarditis in the immunized rabbits. The mean (+/- standard deviation) infectious dose for 50% of the animals was 10(5.29) +/- 10(0.07) in 48 control animals versus 10(7.11) +/- 10(0.22) in 37 immunized rabbits (P less than 0.001). These studies suggest that humoral antibody may protect against C. albicans endocarditis, perhaps through inhibition of adhesion, a crucial early step in the pathogenesis of endocarditis.
通过体外和体内技术研究了预先形成的抗体对实验性白色念珠菌心内膜炎诱导的影响。白色念珠菌与免疫血清（通过静脉注射福尔马林灭活的酵母细胞在兔体内产生）预孵育可降低其在体外对非细菌性血栓性心内膜炎成分（如纤维蛋白加血小板）的黏附。两种不同的方法，使用放射性标记或活酵母细胞，均证实了免疫血清处理的白色念珠菌细胞对对照值的黏附降低至0%至7.8%（P小于0.001）。这些结果与免疫兔免受白色念珠菌心内膜炎的发展相关。48只对照动物中50%动物的平均（±标准差）感染剂量为10(5.29)±10(0.07)，而37只免疫兔中为10(7.11)±10(0.22)（P小于0.001）。这些研究表明，体液抗体可能通过抑制黏附来预防白色念珠菌心内膜炎，黏附是心内膜炎发病机制中的关键早期步骤。
