Traber D L, Adams T, Henriksen N, Traber L D, Thomson P D
J Appl Physiol Respir Environ Exerc Physiol. 1983 Apr;54(4):1167-71. doi: 10.1152/jappl.1983.54.4.1167.
This study compares qualitative and quantitative differences between the cardiopulmonary responses to Salmonella typhimurium and Escherichia coli 055:B5 endotoxins (Difco) in the same sheep model. Lipopolysaccharides, 0.75 micrograms/kg, were infused into chronically instrumented sheep over 30 min. Cardiopulmonary and pulmonary lymph flux data were collected for 2 h prior to and 6 h following this. One week later the sequence was repeated with another endotoxin. The administration of the two endotoxins was varied: some received S. typhimurium first; others received E. coli. A total of 13 animals were studied; 8 had intact pulmonary lymphatic catheters. Following each injection of endotoxin these animals showed a typical response of early high pressure-mediated increase in pulmonary lymph flow and later lymph flow elevation associated with a very mild increase in microvascular pressure and lymph with normal protein levels. These changes were associated with hemoconcentration, lowered arterial oxygen partial pressure, cardiac index, and blood neutrophil count. It is concluded that Difco S. typhimurium and E. coli endotoxins are similar in their mechanism of action and potency and can be studied in the same animal.
本研究在同一绵羊模型中比较了对鼠伤寒沙门氏菌和大肠杆菌055:B5内毒素（Difco）心肺反应的定性和定量差异。将0.75微克/千克的脂多糖在30分钟内输注到长期植入仪器的绵羊体内。在此之前2小时和之后6小时收集心肺和肺淋巴流量数据。一周后，用另一种内毒素重复该顺序。两种内毒素的给药方式有所不同：一些先接受鼠伤寒沙门氏菌；另一些先接受大肠杆菌。总共研究了13只动物；8只有完整的肺淋巴管导管。每次注射内毒素后，这些动物表现出典型的反应，即早期由高压介导的肺淋巴流量增加，随后淋巴流量升高，同时微血管压力和淋巴液有非常轻微的增加，蛋白质水平正常。这些变化与血液浓缩、动脉血氧分压降低、心脏指数和血液中性粒细胞计数降低有关。结论是，Difco鼠伤寒沙门氏菌和大肠杆菌内毒素在作用机制和效力方面相似，可在同一动物中进行研究。
