Ribaux C L, Magloire H, Joffre A, Morrier J J
J Biol Buccale. 1983 Mar;11(1):41-51.
A protein immunologically related to fibronectin was demonstrated on the oral flagellate Trichomonas tenax. Two strains of T. tenax were used, the first freshly taken from ulcerative gingivitis and the second maintained in culture for 2 years. Initial investigations were made by established immunofluorescence staining procedures. Cells of the protozoa gave a positive fluorescence with anti-fibronectin anti-serum, while the controls were negative. Subsequently, indirect immunostaining procedures for electron microscopy were performed using the peroxidase procedure. Peroxidase deposits were detected on the outer layer of the cell membrane. Particularly intense labelling was localized in the contact areas between bacteria and protozoa, and sometimes within the marginal lamina of the undulating membrane. This fibronectin-like protein could potentially play an important role in the adhesion of Trichomonas tenax to gingival cells, connective tissue and in the phagocytosis of bacteria. The attachment of protozoa to gingival substrates could be the first step in the breakdown of periodontal tissues.
在口腔鞭毛虫口腔毛滴虫上发现了一种与纤连蛋白免疫相关的蛋白质。使用了两株口腔毛滴虫，第一株取自溃疡性牙龈炎，第二株在培养物中保存了2年。最初的研究采用既定的免疫荧光染色程序。原生动物细胞用抗纤连蛋白抗血清呈现阳性荧光，而对照组为阴性。随后，使用过氧化物酶程序进行了用于电子显微镜的间接免疫染色程序。在细胞膜外层检测到过氧化物酶沉积物。特别强烈的标记位于细菌与原生动物之间的接触区域，有时位于波动膜的边缘层内。这种纤连蛋白样蛋白可能在口腔毛滴虫与牙龈细胞、结缔组织的黏附以及细菌的吞噬作用中发挥重要作用。原生动物与牙龈底物的附着可能是牙周组织破坏的第一步。
