Observations on the separation of SP1 alpha and SP1 beta by affinity chromatography.

SP1 antigens in late pregnancy blood, in placental extracts and in acidified plasma have been purified on positive affinity chromatography columns with immobilized SP1 antiserum and on negative affinity columns with immobilized antihuman non-pregnancy serum. Both SP1 alpha and SP1 beta are bound by the positive column but only SP1 alpha is bound by the negative column. This is adduced as further support for the view that SP1 alpha is formed by the combination of SP1 beta with a protein present in non-pregnancy serum.