Viklická S, Keprtová J, Tkadlecek L, Cibulka M, Minárová E
Neoplasma. 1978;25(1):13-24.
Uptake of isologous DNA (labeled with 3H-thymidine) by L-cells was investigated by means of electron microscopic autoradiography. It was found that exogenous 3H-DNA occurred not only in the cytoplasm, but, already from the 1st hr of incubation, permeated as far as in the host cell nuclei. During continuous cultivation, radioactivity established in the nuclei and cytoplasm increases with maximum value after 12 hr incubation. Beginning at the 3rd hr of incubation, in cytoplasma, grain clusters over loci of higher radioactivity were found. After 24 hr incubation, similar grain clusters in L-cell nuclei were observed. In the cytoplasma of the cells, the grains were often determined over or in the vicinity of lysosomes; in the nuclei they were localized predominantly over heterochromatine. The manner of 3H-DNA and 3H-TdR labeling was also compared.
通过电子显微镜放射自显影术研究了L细胞对同源DNA（用³H-胸腺嘧啶核苷标记）的摄取。发现外源性³H-DNA不仅存在于细胞质中，而且从孵育的第1小时起就渗透到宿主细胞核中。在连续培养过程中，细胞核和细胞质中建立的放射性在孵育12小时后达到最大值并增加。从孵育的第3小时开始，在细胞质中发现了较高放射性位点上的颗粒簇。孵育24小时后，在L细胞核中观察到类似的颗粒簇。在细胞的细胞质中，颗粒常常在溶酶体上或其附近被确定；在细胞核中，它们主要定位于异染色质上。还比较了³H-DNA和³H-TdR的标记方式。
