Kawaoi A, Okano T, Nemoto N, Shikata T
Acta Endocrinol (Copenh). 1983 Jun;103(2):235-40. doi: 10.1530/acta.0.1030235.
Detection of T4 and T3 in paraffin sections of the rat thyroid gland in various functional states was attempted by an immunoenzyme technique. Both hormones showed a similar localization, being detected mainly in the colloid in the follicular lumen, and sometimes in certain regions of the cytoplasm of the follicular epithelium. Following administration of TSH, their stainability was increased, and the localization within the cytoplasm became more remarkable. Decrease or disappearance of stainability was observed after hypophysectomy or administration of PTU. These findings support the view that the immunostainability of T4 and T3 is closely correlated with the functional state of the thyroid gland.
采用免疫酶技术尝试检测处于不同功能状态的大鼠甲状腺石蜡切片中的T4和T3。两种激素显示出相似的定位，主要在滤泡腔内的胶体中检测到，有时也在滤泡上皮细胞质的某些区域检测到。给予促甲状腺激素（TSH）后，它们的染色性增加，并且在细胞质内的定位变得更加明显。垂体切除或给予丙硫氧嘧啶（PTU）后观察到染色性降低或消失。这些发现支持了T4和T3的免疫染色性与甲状腺的功能状态密切相关的观点。
