Spitalnik S L, Cowles J W, Cox M T, Blumberg N
Am J Clin Pathol. 1983 Jul;80(1):63-5. doi: 10.1093/ajcp/80.1.63.
The neutralization of antibodies to the Lewis blood group systems is important in confirming the presence of these antibodies and in investigating complex alloantibody problems. An improved method for neutralizing Lewis antibodies is described. Insoluble immunoadsorbents containing synthetic Lewis antigens are used to physically remove the antibody from serum. Thirty-five sera containing Lewis antibodies were neutralized completely using this technic; 23 non-Lewis alloantibodies were not inhibited. In contrast with current methods, this technic does not dilute the patient serum and results in an affinity purified serum sample.
对Lewis血型系统抗体的中和在确认这些抗体的存在以及研究复杂的同种抗体问题方面很重要。本文描述了一种改进的Lewis抗体中和方法。含有合成Lewis抗原的不溶性免疫吸附剂用于从血清中物理去除抗体。使用该技术，35份含有Lewis抗体的血清被完全中和；23份非Lewis同种抗体未被抑制。与现有方法相比，该技术不会稀释患者血清，并且可得到亲和纯化的血清样本。
