Chai T J
Appl Environ Microbiol. 1983 Apr;45(4):1316-23. doi: 10.1128/aem.45.4.1316-1323.1983.
Membrane-filtered bay water can support a certain degree of growth of Escherichia coli organisms isolated from the bay water or from sewage. The effect of the growth medium (bay water versus rich medium) on sensitivities to antimicrobial agents and cell envelope proteins was studied in many of these strains. Bay water-grown cells were less sensitive to bacteriophages and colicins, but were more sensitive to heavy metals and detergents as compared with rich-medium-grown cells. These results indicated that the cell envelope composition of the bay water-grown cells could be modified, resulting in altered susceptibility to various antimicrobial agents. An analysis of cell envelope proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that cells from rich-medium-grown cultures contained two or three major outer membrane proteins, whereas in bay water-grown cells, the OmpF protein was greatly reduced.
经膜过滤的海湾水能够支持从海湾水或污水中分离出的大肠杆菌一定程度的生长。在许多这些菌株中，研究了生长培养基（海湾水与丰富培养基）对抗菌剂敏感性和细胞包膜蛋白的影响。与在丰富培养基中生长的细胞相比，在海湾水中生长的细胞对噬菌体和大肠杆菌素不太敏感，但对重金属和洗涤剂更敏感。这些结果表明，在海湾水中生长的细胞的细胞膜组成可能会发生改变，从而导致对各种抗菌剂的敏感性发生变化。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳对细胞膜蛋白进行分析表明，在丰富培养基中生长的培养物中的细胞含有两到三种主要的外膜蛋白，而在海湾水中生长的细胞中，外膜蛋白F大大减少。
