普氏立克次体柠檬酸合酶基因在大肠杆菌中的表达。
Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli.
作者信息
Wood D O, Atkinson W H, Sikorski R S, Winkler H H
出版信息
J Bacteriol. 1983 Jul;155(1):412-6. doi: 10.1128/jb.155.1.412-416.1983.
Abstract
Recombinant DNA techniques were used to isolate the Rickettsia prowazekii citrate synthase gene on the plasmid vector pBR322 by functional complementation of a gltA mutation of Escherichia coli K-12. Analysis of citrate synthase activity in crude extracts revealed that the enzyme expressed in E. coli retains the regulatory control mechanisms characteristic of the rickettsial enzyme.
摘要
通过对大肠杆菌K-12的gltA突变进行功能互补，利用重组DNA技术在质粒载体pBR322上分离出普氏立克次体柠檬酸合酶基因。对粗提取物中柠檬酸合酶活性的分析表明，在大肠杆菌中表达的该酶保留了立克次体酶特有的调控机制。
Zotero 插件