Cook D L
Metabolism. 1983 Jul;32(7):681-5. doi: 10.1016/0026-0495(83)90124-5.
Glucose-induced membrane electrical activity was recorded from single isolated mouse islets of Langerhans exposed to steady levels or to step changes of glucose concentration. Superimposed on the well-known rapid (approximately 15-second period) alternations in membrane potential, slow oscillations in the intensity of the electrical activity were observed having a period of 4.6 +/- 0.2 minute (mean +/- SEM, n = 19 islets) and a range of 3.0-6.2 minutes. The largest observed amplitude of oscillation was nearly 50% of the mean intensity. In 62 consecutive recordings from different islets, eleven (18%) islets oscillated steadily after 10 minutes of constant bath conditions while an additional eight islets (13%) oscillated only transiently following abrupt changes of islet stimulation. The oscillations, when triggered by changes of islet stimulation, appeared to be a "ringing" of the biphasic kinetics previously described for both electrical activity and insulin release. Since glucose-induced electrical activity is known to be well correlated with insulin secretory rate, these observations suggest that single isolated mouse islets may also display periodic insulin secretion.
在暴露于稳定水平或葡萄糖浓度阶跃变化的单个分离小鼠胰岛中记录葡萄糖诱导的膜电活动。叠加在膜电位众所周知的快速（约15秒周期）交替变化上，观察到电活动强度的缓慢振荡，其周期为4.6±0.2分钟（平均值±标准误，n = 19个胰岛），范围为3.0 - 6.2分钟。观察到的最大振荡幅度接近平均强度的50%。在来自不同胰岛的62次连续记录中，11个（18%）胰岛在恒定浴条件10分钟后稳定振荡，另外8个胰岛（13%）仅在胰岛刺激突然变化后短暂振荡。当由胰岛刺激变化触发时，这些振荡似乎是先前描述的电活动和胰岛素释放双相动力学的“回响”。由于已知葡萄糖诱导的电活动与胰岛素分泌率密切相关，这些观察结果表明单个分离的小鼠胰岛也可能表现出周期性胰岛素分泌。
