Scott A M, Atwater I, Rojas E
Diabetologia. 1981 Nov;21(5):470-5. doi: 10.1007/BF00257788.
A method has been developed for the simultaneous measurement of insulin release and electrical activity in single micro-dissected mouse islets of Langerhans. The effects of D-glucose have been studied in individual islets. Each islet was exposed to 0, 5.6, 11.1, 16.7, 22.2, 27.8, and 33.3 mmol/l glucose in a stepwise fashion. The minimum glucose concentration required to elicit spike activity is lower than that required to stimulate insulin release above basal levels and the maximum spike frequency occurs at a lower glucose concentration than does maximum insulin release. Following a reduction in glucose from 27,8 (or 33.3) to 5.6 mmol/l, membrane potentials returned to resting values within 2 min whereas insulin returned to basal values after 20 min. Increasing glucose from 5.6 to 27.8 mmol/l induced spike activity within 10 s; the insulin response was detected within 40 s. Thus, it is possible to use the single mouse islet for simultaneous measurements of insulin release and electrical activity.
已开发出一种用于同时测量单个显微解剖的小鼠胰岛中胰岛素释放和电活动的方法。已在单个胰岛中研究了D-葡萄糖的作用。每个胰岛以逐步方式暴露于0、5.6、11.1、16.7、22.2、27.8和33.3 mmol/l的葡萄糖中。引发峰电位活动所需的最低葡萄糖浓度低于刺激胰岛素释放高于基础水平所需的浓度，并且最大峰频率出现在比最大胰岛素释放更低的葡萄糖浓度下。在将葡萄糖从27.8（或33.3）mmol/l降至5.6 mmol/l后，膜电位在2分钟内恢复到静息值，而胰岛素在20分钟后恢复到基础值。将葡萄糖从5.6 mmol/l增加到27.8 mmol/l在10秒内诱导峰电位活动；胰岛素反应在40秒内被检测到。因此，有可能使用单个小鼠胰岛同时测量胰岛素释放和电活动。
