Sherwood J B, Shouval D
Proc Natl Acad Sci U S A. 1986 Jan;83(1):165-9. doi: 10.1073/pnas.83.1.165.
Establishment of a stable, transformed human renal carcinoma cell line that produces erythropoietin in vitro and has maintained this function continuously since 1981 and for greater than 150 passages in monolayer culture was accomplished by transplantation of human renal clear cell carcinoma tissue from a patient with erythrocytosis into an immunosuppressed athymic mouse. In addition to its immunocrossreactivity with native human urinary erythropoietin, the tumor erythropoietin demonstrates biological activity in the in vitro mouse erythroid colony-forming unit assay and in tumor-bearing nude mice. The cloned renal carcinoma cell line has an abnormal human karyotype and has ultrastructural features characteristic of human renal clear cell carcinoma. This cell line provides a reproducible model system for the production of an erythropoietin-like material and for the study of its synthesis and secretion.
通过将一名红细胞增多症患者的人肾透明细胞癌组织移植到免疫抑制的无胸腺小鼠体内，建立了一种稳定的、经过转化的人肾癌细胞系。该细胞系自1981年以来一直在体外产生促红细胞生成素，并且在单层培养中连续传代超过150次仍保持这一功能。除了与天然人尿促红细胞生成素具有免疫交叉反应性外，肿瘤促红细胞生成素在体外小鼠红系集落形成单位试验以及荷瘤裸鼠中均表现出生物活性。该克隆的肾癌细胞系具有异常的人类核型，并具有人肾透明细胞癌的超微结构特征。该细胞系为生产促红细胞生成素样物质及其合成和分泌的研究提供了一个可重复的模型系统。
