脂肪细胞葡萄糖代谢及脂肪酸释放对腺苷脱氨酶、胰岛素和灌流的反应。通过灌流研究中间代谢。
The response of adipocyte glucose metabolism and fatty acid release to adenosine deaminase, insulin and perifusion. Investigation of intermediary metabolism by perifusion.
作者信息
Harper R D
机构信息
Faculty of Applied Sciences, Luton College of Higher Education, Bedfordshire, U.K.
出版信息
Biochem J. 1988 May 1;251(3):733-7. doi: 10.1042/bj2510733.
Abstract
Adipocytes incubated with adenosine deaminase (ADA) showed: (1) increased amounts of fatty acids in the medium; (2) increased glucose incorporation into acylglycerol glycerol; (3) decreased glucose incorporation into acylglycerol fatty acids; (4) a co-ordinate decrease in the sensitivity of lipolysis and glucose incorporation into acylglycerol to insulin; (5) similar effects on glucose incorporations in perifused and normal incubations. The decrease in fatty acid synthesis by perfusion was found to be dependent on the presence of insulin or fatty acids, and independent of the effects of ADA. The significance of the effects of perifusion, ADA and insulin are discussed in relation to effects of fatty acids.
摘要
与腺苷脱氨酶（ADA）一起孵育的脂肪细胞表现出：（1）培养基中脂肪酸含量增加；（2）葡萄糖掺入酰基甘油甘油的量增加；（3）葡萄糖掺入酰基甘油脂肪酸的量减少；（4）脂解和葡萄糖掺入酰基甘油对胰岛素的敏感性协同降低；（5）在灌流和正常孵育中对葡萄糖掺入有类似影响。发现通过灌流脂肪酸合成的减少取决于胰岛素或脂肪酸的存在，且与ADA的作用无关。结合脂肪酸的作用讨论了灌流、ADA和胰岛素作用的意义。
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A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.一种用于分离脂肪细胞的改良灌流培养系统。通过灌流研究中间代谢。
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Fatty acids reverse the cyclic AMP inhibition of triacylglycerol and phosphatidylcholine synthesis in rat hepatocytes.脂肪酸可逆转环磷酸腺苷对大鼠肝细胞中三酰甘油和磷脂酰胆碱合成的抑制作用。
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