Seymour A M, Bailey I A, Radda G K
Biochim Biophys Acta. 1983 Jul 14;762(4):525-30. doi: 10.1016/0167-4889(83)90056-3.
The effect on the recovery of mechanical function, ATP, phosphocreatine, Pi and pH of various lengths of total global ischaemia in the insulin-treated, perfused rat heart has been studied using 31P-NMR. Insulin-treated hearts recovered stable mechanical function after 18 min ischaemia when their intracellular pH was 6.0 and 70% of the pre-ischaemic ATP remained. Hearts perfused without insulin fail to recover after 18 min ischaemia, having an intracellular pH of 6.3 and 40% of ATP remaining (Bailey, I.A., Seymour, A.-M.L. and Radda, G.K. (1981) Biochim, Biophys. Acta 637, 1-7). Thus, ATP maintenance in ischaemia is more important to recovery on reperfusion than is maintaining intracellular pH. The importance of this observation in devising biochemical strategies for the clinical protection of the myocardium is discussed.
利用31P-NMR研究了胰岛素处理的灌注大鼠心脏中不同时长的完全性全心缺血对机械功能恢复、ATP、磷酸肌酸、无机磷和pH值的影响。胰岛素处理的心脏在缺血18分钟后恢复了稳定的机械功能，此时其细胞内pH值为6.0，且缺血前ATP的70%仍然存在。未用胰岛素灌注的心脏在缺血18分钟后未能恢复，其细胞内pH值为6.3，ATP剩余40%（贝利，I.A.，西摩，A.-M.L.和拉达，G.K.（1981年）《生物化学与生物物理学学报》637，1-7）。因此，缺血时ATP的维持对再灌注后的恢复比维持细胞内pH值更为重要。讨论了这一观察结果在设计心肌临床保护生化策略中的重要性。
