Hattori T, Pack M, Bougnoux P, Chang Z L, Hoffman T
J Clin Invest. 1983 Jul;72(1):237-44. doi: 10.1172/jci110962.
Effects of human fibroblast (beta) or leukocyte (alpha) interferon (IFN) on differentiations of a human histiocytic lymphoma-derived cell line (U937) or promyelocytic leukemia-derived cell line (HL-60) were studied. When cultured with beta-IFN (400-1,000 U/ml), U937 cells showed gross morphologic and microscopic changes consisting of clumping, increased cytoplasmic-to-nuclear ratio, enhanced prominence of cytoplasmic granules, and membrane ruffling. After culture with beta-IFN, the number of U937 cells reactive with B43.4.1 monoclonal antibody, which is specific for human monocytes, natural killer cells, and neutrophils, increased from less than 10% of U937 cells to 47% beta-IFN treatment also enhanced antibody-dependent cellular cytotoxicity against chicken erythrocytes by U937 cells. The same morphologic, phenotypic, and functional changes were also observed when U937 were treated with recombinant or natural alpha-IFN. The effects of alpha-IFN were totally abolished by anti-alpha-IFN serum. In contrast, HL-60, which differentiates toward cells of the monocyte lineage in response to phorbol 12-myristate 13-acetate (based on the above criteria), and toward granulocytes in response to dimethyl sulfoxide, did not differentiate when cultured with alpha- or beta-IFN. No consistent relationship between induction of differentiation and changes in phospholipid methylation were observed.
研究了人成纤维细胞（β）干扰素或白细胞（α）干扰素对人组织细胞淋巴瘤来源的细胞系（U937）或早幼粒细胞白血病来源的细胞系（HL-60）分化的影响。当与β干扰素（400 - 1000 U/ml）一起培养时，U937细胞出现了明显的形态学和显微镜下变化，包括细胞聚集、细胞质与细胞核比例增加、细胞质颗粒突出增强以及细胞膜褶皱。用β干扰素培养后，与对人单核细胞、自然杀伤细胞和中性粒细胞具有特异性的B43.4.1单克隆抗体反应的U937细胞数量从U937细胞的不到10%增加到47%。β干扰素处理还增强了U937细胞对鸡红细胞的抗体依赖性细胞毒性。当用重组或天然α干扰素处理U937细胞时，也观察到了相同的形态学、表型和功能变化。α干扰素的作用被抗α干扰素血清完全消除。相比之下，HL-60细胞在佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（基于上述标准）作用下向单核细胞系细胞分化，在二甲基亚砜作用下向粒细胞分化，但在用α或β干扰素培养时未发生分化。未观察到分化诱导与磷脂甲基化变化之间的一致关系。
