Donnini C, Puglisi P P, Marmiroli N
Mol Gen Genet. 1983;190(3):504-10. doi: 10.1007/BF00331083.
The accumulation of respiratory deficient (RD) mutants in Saccharomyces cerevisiae depended upon the mutagens used and upon the presence of the nuclear gene previously identified as MMC1 (one) which we showed to control the spontaneous and the erythromycin-induced RD mutability. In this paper data are reported about the accumulation of RD mutants in the presence of manganous ions (Mn++) and UV which was higher in the mmc1 (one) than in MMC1 strains. We found that the characters 'low spontaneous' and 'low induced' RD mutability by erythromycin, manganous ions and UV, were controlled by the same genetic determinant. In the presence of manganous ions, also the frequency of antibiotic resistant mutants capR and eryR was higher in the mmc1 strains. Moreover, the accumulation of RD mutants in the presence of berenil, 5-fluorouracil and basic fuchsin was higher in the mmc1 than in MMC1 strains. In contrast, RD mutants accumulation by acriflavine and ethidium bromide treatments did not appear affected by the MMC1 genetic constitution.
酿酒酵母中呼吸缺陷（RD）突变体的积累取决于所使用的诱变剂以及先前鉴定为MMC1（一个）的核基因的存在，我们已证明该基因可控制自发的和红霉素诱导的RD突变率。本文报道了在存在锰离子（Mn++）和紫外线的情况下RD突变体的积累情况，mmc1（一个）菌株中的积累高于MMC1菌株。我们发现，红霉素、锰离子和紫外线导致的“低自发”和“低诱导”RD突变率特征由相同的遗传决定因素控制。在存在锰离子的情况下，mmc1菌株中抗生素抗性突变体capR和eryR的频率也更高。此外，在存在贝尼尔、5-氟尿嘧啶和碱性品红的情况下，mmc1菌株中RD突变体的积累高于MMC1菌株。相比之下，吖啶黄素和溴化乙锭处理导致的RD突变体积累似乎不受MMC1遗传构成的影响。
