Connolly T P, Sproule S D, Man S F
Clin Invest Med. 1983;6(2):89-95.
We have devised a technique for collecting airway fluid from the bronchi in order to investigate the biochemical indices of airway fluid in normal dogs and the changes produced in them by pharmacological interventions. We collected airway fluid from the trachea by a screen technique and by means of filter paper cylinders inserted through a fiberoptic bronchoscope (the new method) and from main and segmental bronchi by the new method only. When bronchial fluid was compared with plasma, the concn of albumin was found to be much lower, Na+ lower, and Cl- either higher or similar. The Na+:Cl- ratio was lowest in fluid from the trachea (1.04) and highest in fluid from segmental bronchi (1.14), but was significantly lower at all levels than in plasma (1.32). Subcutaneous administration of methacholine chloride significantly increased the weight of airway fluid collections. Infusion of 5% hypertonic saline induced changes in the concns of electrolytes (but not of albumin) that correlated with changes in the plasma. The major differences in electrolyte and albumin concns in airway fluid and plasma likely reflect a bidirectional transepithelial flux of these ions. The regional differences in electrolyte concns and ratios may be due to differences in anatomic structure and in the functioning of ionic pumps along the tracheobronchial tree.
我们设计了一种从支气管收集气道液体的技术，以研究正常犬气道液体的生化指标以及药物干预对其产生的变化。我们通过滤网技术并借助插入纤维支气管镜的滤纸圆筒（新方法）从气管收集气道液体，且仅通过新方法从主支气管和段支气管收集气道液体。当将支气管液体与血浆进行比较时，发现白蛋白浓度低得多，钠离子浓度较低，氯离子浓度要么较高要么相似。钠离子与氯离子的比率在气管液体中最低（1.04），在段支气管液体中最高（1.14），但在所有水平上均显著低于血浆中的比率（1.32）。皮下注射氯化乙酰甲胆碱显著增加了气道液体收集量。输注5%高渗盐水引起电解质浓度（但不是白蛋白浓度）的变化，这些变化与血浆中的变化相关。气道液体和血浆中电解质和白蛋白浓度的主要差异可能反映了这些离子的双向跨上皮通量。电解质浓度和比率的区域差异可能是由于气管支气管树的解剖结构和离子泵功能的差异所致。
