Reasor M J, Cohen D, Proctor D F, Rubin R J
J Appl Physiol Respir Environ Exerc Physiol. 1978 Aug;45(2):190-4. doi: 10.1152/jappl.1978.45.2.190.
The effect of cholinomimetic stimulation on the quantity and composition of tracheobronchial secretions (TBS) collected from intact dogs was studied. After the administration of methacholine chloride, the collection rate of TBS increased over threefold within 10 min and returned to the control level by 50 min. Even though there was an increase in the total amount of protein collected, the protein concentration of the secretions decreased initially by 40%, returning to normal by 30 min. This was the result of an apparent dilution of the secretions by nonproteinaceous components. In addition, at 10 min, there was an increase in the protein associated with mucins. These responses were blocked by the preadministration of atropine. When TBS was separated into supernatant and pellet phases by centrifugation, we found that, following methacholine, there was a 35% increase in the ratio of albumin to IgG in the supernatant phase. These results suggest that the increased secretion due to methacholine may result, in part, by a selective filtration of fluid from the interstitial and/or vascular compartment(s) with proteins appearing in the secretory mixture based partially on their size.
研究了拟胆碱刺激对从完整犬收集的气管支气管分泌物（TBS）的量和成分的影响。给予氯化乙酰甲胆碱后，TBS的收集速率在10分钟内增加了三倍多，并在50分钟时恢复到对照水平。尽管收集的蛋白质总量有所增加，但分泌物的蛋白质浓度最初下降了40%，在30分钟时恢复正常。这是由于非蛋白质成分对分泌物的明显稀释所致。此外，在10分钟时，与粘蛋白相关的蛋白质增加。这些反应被预先给予阿托品所阻断。当通过离心将TBS分离为上清相和沉淀相时，我们发现，给予乙酰甲胆碱后，上清相中白蛋白与IgG的比例增加了35%。这些结果表明，乙酰甲胆碱引起的分泌物增加可能部分是由于液体从间质和/或血管腔室选择性滤过，分泌混合物中的蛋白质部分基于其大小而出现。
