Tung E, Goust J M, Chen W Y, Kang S S, Wang I Y, Wang A C
Immunology. 1983 Sep;50(1):57-64.
The secreted IgMs of two human lymphoblastoid cell lines, RPMI-6410 and RPMI-8392, were purified. Antisera against these two IgMs were raised in rabbits and made idiotypically specific to the respective antigens through various absorption procedures. By immunofluorescence and radioimmunoassay techniques, the purified anti-idiotype antibodies were found to react also with the membrane Igs of the respective cell lines, but not with those of other cell lines. The purified anti-idiotype antibodies were then coupled with Chlorambucil to form antibody-drug conjugates, whose effectiveness in the in-vitro killing of target cells was evaluated by a chromium-release cytotoxicity assay. The results showed that these anti-idiotype antibody-Chlorambucil conjugates were specifically cytotoxic to lymphoblastoid cells that bore membrane Igs carrying the respective idiotypic determinant(s). Furthermore, the conjugates were far more effective in causing cytolysis to the target cells than either Chlorambucil or the anti-idiotype antibodies alone.
纯化了两个人类淋巴母细胞系RPMI - 6410和RPMI - 8392分泌的IgM。在兔子体内制备了针对这两种IgM的抗血清，并通过各种吸收程序使其对各自的抗原具有独特型特异性。通过免疫荧光和放射免疫测定技术发现，纯化的抗独特型抗体也能与各自细胞系的膜Ig发生反应，但不与其他细胞系的膜Ig反应。然后将纯化的抗独特型抗体与苯丁酸氮芥偶联形成抗体 - 药物偶联物，通过铬释放细胞毒性试验评估其在体外杀伤靶细胞的有效性。结果表明，这些抗独特型抗体 - 苯丁酸氮芥偶联物对携带各自独特型决定簇的膜Ig的淋巴母细胞具有特异性细胞毒性。此外，与单独使用苯丁酸氮芥或抗独特型抗体相比，偶联物在引起靶细胞细胞溶解方面更有效。
