遗传性补体第五成分缺陷小鼠对金黄色葡萄球菌的肺部防御功能受损。
Impaired lung defenses against Staphylococcus aureus in mice with hereditary deficiency of the fifth component of complement.
作者信息
Cerquetti M C, Sordelli D O, Ortegon R A, Bellanti J A
出版信息
Infect Immun. 1983 Sep;41(3):1071-6. doi: 10.1128/iai.41.3.1071-1076.1983.
Mice of the C5-deficient DBA/2J and B10.D2/oSnJ inbred strains were aerosolized with Staphylococcus aureus, and the pulmonary clearance of bacteria was determined 4 h later. Both C5-deficient strains had a significantly decreased lung clearance of S. aureus compared with their genetically closest C5-sufficient relatives, DBA/1J and B10.D2/nSnJ strains, respectively. Serum hemolytic activity and pulmonary clearance of S. aureus were also investigated in F1 and F2 progenies (DBA/1J X DBA/2J and DBA/2J X B10.D2/oSnJ). Serum hemolytic activity was present in all F1 (DBA/1J X DBA/2J) mice, and their pulmonary clearance of S. aureus was no different from that of the C5-sufficient parents (DBA/1J). The absence of serum hemolytic activity (absence of C5) in all mice from F1 and F2 (DBA/2J X B10.D2/oSnJ) and 20% of the F2 (DBA/1J X DBA/2J) was related to a decreased lung clearance of S. aureus. These results are consistent with an autosomal recessive pattern of heredity for the murine abnormality in pulmonary clearance of S. aureus.
将金黄色葡萄球菌雾化接种于C5缺陷的近交系DBA/2J和B10.D2/oSnJ小鼠，4小时后测定肺部细菌清除率。与它们基因关系最密切的C5充足的亲属DBA/1J和B10.D2/nSnJ品系相比，这两个C5缺陷品系的金黄色葡萄球菌肺部清除率均显著降低。还对F1和F2后代（DBA/1J×DBA/2J和DBA/2J×B10.D2/oSnJ）的血清溶血活性和金黄色葡萄球菌肺部清除率进行了研究。所有F1（DBA/1J×DBA/2J）小鼠均有血清溶血活性，其金黄色葡萄球菌肺部清除率与C5充足的亲本（DBA/1J）无差异。F1和F2（DBA/2J×B10.D2/oSnJ）的所有小鼠以及20%的F2（DBA/1J×DBA/2J）小鼠缺乏血清溶血活性（缺乏C5）与金黄色葡萄球菌肺部清除率降低有关。这些结果与小鼠金黄色葡萄球菌肺部清除异常的常染色体隐性遗传模式一致。
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