大肠杆菌溶血特性与铁代谢之间的关系
Relation between the hemolytic property and iron metabolism in Escherichia coli.
作者信息
Lebek G, Gruenig H M
出版信息
Infect Immun. 1985 Dec;50(3):682-6. doi: 10.1128/iai.50.3.682-686.1985.
Abstract
The hemolytic activity of wild-type strains of Escherichia coli was measured by a standardized method in liquid broth. The system also allowed us to investigate the influence of various Fe3+ concentrations in the cultures on the amount of secreted hemolysin. We found that the hemolysin secretion of all strains was clearly reduced after addition of FeCl3. However, the influence of additional iron chelators showed remarkable differences. The hemolytic activity of Hly plasmid-containing strains isolated in Berne significantly increased. Most of the strains with a chromosomal hemolysin determinant showed a similar effect, but to a lesser degree. Contrary to this, Hly plasmid-containing strains isolated in Essex and some strains with chromosomal hemolysin determinant were either not affected or even showed reduced hemolysin secretion after limitation of free iron ions in the broth. Our results suggest that hemolysin secretion in E. coli is related to the bacterial iron metabolism, and hemolysin secretion is differentially regulated among E. coli strains.
摘要
通过标准化方法在液体肉汤中测定大肠杆菌野生型菌株的溶血活性。该系统还使我们能够研究培养物中不同Fe3+浓度对分泌的溶血素量的影响。我们发现添加FeCl3后所有菌株的溶血素分泌明显减少。然而，额外铁螯合剂的影响显示出显著差异。在伯尔尼分离的含Hly质粒的菌株的溶血活性显著增加。大多数具有染色体溶血素决定簇的菌株表现出类似的效果，但程度较小。与此相反，在埃塞克斯分离的含Hly质粒的菌株和一些具有染色体溶血素决定簇的菌株在肉汤中游离铁离子受限后要么不受影响，甚至溶血素分泌减少。我们的结果表明，大肠杆菌中的溶血素分泌与细菌铁代谢有关，并且溶血素分泌在大肠杆菌菌株之间受到不同的调节。
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