大鼠C6胶质瘤细胞增殖的控制：氢化可的松激素在悬浮培养和单层培养中抑制作用的解偶联。
Control of rat C6 glioma cell proliferation: uncoupling of the inhibitory effects of hydrocortisone hormone in suspension and monolayer cultures.
作者信息
Armelin M C, Stocco R C, Armelin H A
出版信息
J Cell Biol. 1983 Aug;97(2):455-8. doi: 10.1083/jcb.97.2.455.
Abstract
We undertook a comparative study of the effects of the hormone hydrocortisone (Hy) on C6 glioma cells grown in monolayer and in suspension in cultures. We found Hy reversibly renders C6 cells anchorage- and serum-dependent for their growth. In monolayer cultures, Hy was found to inhibit cell cycle traversing exclusively at G1 phase. In agarose suspension, Hy was found to block colony development. Hy-resistant variants were selected and isolated in agarose suspension. Examination of these variants showed that cells selected for Hy-resistance in suspension can be Hy sensitive when anchored to a solid substrate. We conclude that resistance to Hy in suspension and resistance to it in monolayer culture are two independent phenotypes.
摘要
我们对激素氢化可的松（Hy）对单层培养和悬浮培养的C6胶质瘤细胞的影响进行了一项比较研究。我们发现Hy能使C6细胞的生长可逆地依赖于贴壁和血清。在单层培养中，发现Hy仅在G1期抑制细胞周期进程。在琼脂糖悬浮培养中，发现Hy能阻止集落形成。在琼脂糖悬浮培养中选择并分离出了Hy抗性变体。对这些变体的检测表明，在悬浮培养中选择的Hy抗性细胞在锚定到固体基质上时可能对Hy敏感。我们得出结论，悬浮培养中对Hy的抗性和单层培养中对Hy的抗性是两种独立的表型。
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本文引用的文献
1
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.用于多瘤病毒转化细胞选择性测定的琼脂悬浮培养
Virology. 1964 Jun;23:291-4. doi: 10.1016/0042-6822(64)90301-0.
2
Serum-free cell culture: a unifying approach.无血清细胞培养：一种统一方法。
Cell. 1980 Dec;22(3):649-55. doi: 10.1016/0092-8674(80)90540-1.
3
Glucocorticoid hormone renders rat glioma cells dependent on high concentrations of external Ca2+ for growth.糖皮质激素使大鼠胶质瘤细胞生长依赖于高浓度的细胞外钙离子。
J Cell Physiol. 1983 Apr;115(1):99-104. doi: 10.1002/jcp.1041150115.
4
Pituitary fibroblast growth factors: partial purification and characterization.垂体成纤维细胞生长因子：部分纯化与特性鉴定
Braz J Med Biol Res. 1981 Apr;14(1):19-27.
5
Purification and partial characterization of an acidic fibroblast growth factor from bovine pituitary.从牛垂体中纯化酸性成纤维细胞生长因子并进行部分特性鉴定。
J Biol Chem. 1982 Aug 25;257(16):9692-7.
6
"Contact inhibition" of cell division in 3T3 cells.3T3细胞中细胞分裂的“接触抑制”
Proc Natl Acad Sci U S A. 1968 May;60(1):300-4. doi: 10.1073/pnas.60.1.300.
7
Differentiated rat glial cell strain in tissue culture.组织培养中的分化大鼠神经胶质细胞系。
Science. 1968 Jul 26;161(3839):370-1. doi: 10.1126/science.161.3839.370.
8
Effect of catecholamines on the adenosine 3':5'-cyclic monophosphate concentrations of clonal satellite cells of neurons.儿茶酚胺对神经元克隆卫星细胞中环磷酸腺苷3':5'-浓度的影响。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2165-8. doi: 10.1073/pnas.68.9.2165.
9
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Nature. 1974 May 10;249(453):123-7. doi: 10.1038/249123a0.
10
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Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6. doi: 10.1073/pnas.70.9.2702.
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