胶原蛋白基质促进胰腺内分泌细胞单层重组成胰岛样类器官。
Collagen matrix promotes reorganization of pancreatic endocrine cell monolayers into islet-like organoids.
作者信息
Montesano R, Mouron P, Amherdt M, Orci L
出版信息
J Cell Biol. 1983 Sep;97(3):935-9. doi: 10.1083/jcb.97.3.935.
Abstract
To evaluate the capacity of pancreatic endocrine cells to reassociate in vitro according to the characteristic topographical pattern observed in the islets of Langerhans in situ, we cultured cells dissociated from neonatal rat pancreas within a three-dimensional collagen matrix. Cell monolayers grown on the surface of collagen gels were covered with a second layer of collagen. This induced the monolayers of endocrine cells to reorganize into smooth-contoured, three-dimensional aggregates, in which non-B cells (identified by electron microscopy and immunofluorescence) had a preferential distribution at the periphery, whereas B cells were concentrated in a central position. These results show that cultured pancreatic endocrine cells have the capacity to reassociate into islet-like organoids in vitro, and that collagen matrices may have a permissive effect on the expression of this potential.
摘要
为了根据在原位胰岛中观察到的特征性拓扑模式评估胰腺内分泌细胞在体外重新结合的能力，我们在三维胶原基质中培养了从新生大鼠胰腺分离的细胞。在胶原凝胶表面生长的细胞单层被第二层胶原覆盖。这促使内分泌细胞单层重新组织成轮廓光滑的三维聚集体，其中非B细胞（通过电子显微镜和免疫荧光鉴定）在外围有优先分布，而B细胞集中在中心位置。这些结果表明，培养的胰腺内分泌细胞在体外有能力重新结合成胰岛样类器官，并且胶原基质可能对这种潜能的表达有促进作用。
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本文引用的文献
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Influence of collagen gel on the orientation of epithelial cell polarity: follicle formation from isolated thyroid cells and from preformed monolayers.胶原凝胶对上皮细胞极性取向的影响：从分离的甲状腺细胞和预先形成的单层细胞形成滤泡。
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Collagen as a substrate for cell growth and differentiation.胶原蛋白作为细胞生长和分化的基质。
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Three-dimensional growth and morphogenesis of mouse submandibular epithelial cells in serum-free primary culture.无血清原代培养中小鼠下颌下腺上皮细胞的三维生长与形态发生
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Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture.上皮细胞系对胶原蛋白覆盖的反应形成管腔：一种培养中的形态发生模型。
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Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels.包埋于胶原凝胶中的正常乳腺上皮细胞原代培养中的持续生长。
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Association between tension and orientation of periodontal ligament fibroblasts and exogenous collagen fibres in collagen gels in vitro.体外胶原凝胶中牙周膜成纤维细胞的张力与取向及外源性胶原纤维之间的关联
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Contraction and organization of collagen gels by cells cultured from periodontal ligament, gingiva and bone suggest functional differences between cell types.来自牙周韧带、牙龈和骨组织培养的细胞对胶原凝胶的收缩和组织作用表明了不同细胞类型之间的功能差异。
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