Gaunt S J
J Embryol Exp Morphol. 1983 Jun;75:259-70.
The rat sperm surface antigen 2D6, located over the entire surface of the spermatozoon, is shown by use of a monoclonal antibody in indirect immunofluorescence experiments to spread laterally over the surface of the egg after fusion of sperm and egg plasma membranes at fertilization. Freshly fertilized eggs, obtained from superovulated rats 14 h after hCG injection, showed the 2D6 antigen to have spread in a gradient over a discrete fan-shaped area of the egg surface anterior to the protruding sperm tail. Eggs at a later stage of sperm incorporation, obtained 20 h after hCG injection, showed that the spread of antigen had extended to cover most or all of their surfaces. By 40 h after hCG injection, the approximate time that fertilized eggs cleaved to form 2-cell embryos, most of the 2D6 antigen had been lost from the cell surface. Fertilized eggs, but not unfertilized eggs or 2-cell embryos, were lysed by 2D6 monoclonal antibody in the presence of guinea pig complement. A model for sperm-egg fusion is presented to account for the observed pattern of spreading shown by the 2D6 antigen. The possible role of sperm antigens on the egg surface is discussed.
大鼠精子表面抗原2D6位于精子的整个表面，在间接免疫荧光实验中使用单克隆抗体显示，在受精时精子与卵质膜融合后，该抗原在卵表面横向扩散。从注射hCG 14小时后的超排卵大鼠获得的新鲜受精卵显示，2D6抗原在突出的精子尾部前方的卵表面离散扇形区域呈梯度扩散。在注射hCG 20小时后获得的精子纳入后期的卵显示，抗原的扩散已扩展到覆盖其大部分或全部表面。到注射hCG后40小时，即受精卵分裂形成2细胞胚胎的大致时间，大部分2D6抗原已从细胞表面消失。在豚鼠补体存在的情况下，2D6单克隆抗体可裂解受精卵，但不能裂解未受精卵或2细胞胚胎。本文提出了一个精卵融合模型，以解释2D6抗原所显示的观察到的扩散模式。讨论了精子抗原在卵表面的可能作用。
