少量的序列异质性会降低质粒与酿酒酵母染色体的重组。
Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.
作者信息
Smolik-Utlaut S, Petes T D
出版信息
Mol Cell Biol. 1983 Jul;3(7):1204-11. doi: 10.1128/mcb.3.7.1204-1211.1983.
Abstract
As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.
摘要
作为研究酿酒酵母有丝分裂重组特性的模型系统，我们检测了重组质粒（通过转化导入酿酒酵母细胞）与同源染色体位点之间的重组。这些实验中使用的重组质粒含有酿酒酵母rRNA基因。我们发现，整合重组频率对少量序列异质性敏感。此外，这些重组事件的频率和特异性受相互作用的同源DNA序列长度的影响。
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本文引用的文献
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