Klein H L, Petes T D
Mol Cell Biol. 1984 Feb;4(2):329-39. doi: 10.1128/mcb.4.2.329-339.1984.
We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.
我们利用转化作用，将一个可选择标记插入酿酒酵母基因组中被转座因子Ty占据的各个位点。载体CV9含有LEU2⁺基因和重复元件Ty1 - 17的一部分。用该质粒进行转化，通过在LEU2位点或分散于酿酒酵母基因组中的各种Ty元件处利用同源性进行相互交换，从而导致载体整合。这些转化体被用于对几个Ty元件的位点进行遗传定位。在Ty位点回收的24个转化体定义了19个不同的位点。其中7个被定位到遗传图谱上。在这些实验中鉴定出两类Ty元件：Ty1 - 17类和不同于Ty1 - 17的Ty元件。对每类Ty元件处转化体数量的统计分析表明，CV9质粒优先整合到Ty1 - 17类中。
