Parry J B, Stewart J C, Heptinstall J
Biochem J. 1983 Aug 1;213(2):437-44. doi: 10.1042/bj2130437.
Aspergillus fumigatus (Fresenius), IMI 246651, A.T.C.C. 46324, produces two beta-glucosidase enzymes, cotton-solubilizing activity, xylanase and endoglucanase enzymes which can be separated by gel-filtration chromatography. The major endoglucanase does not bind to concanavalin A-Sepharose and does not stain with periodic acid/Schiff reagent. It is homogeneous on polyacrylamide isoelectric focusing (pI = 7.1) and has a mol.wt. of 12500 by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The endoglucanase produces glucose and a mixture of oligosaccharides from cellulose; the purified enzyme has a small dextranase activity. It is stable at 50 degrees C and pH 6.
烟曲霉（弗里斯），IMI 246651，美国典型培养物保藏中心46324号，产生两种β-葡萄糖苷酶、棉纤维溶解活性、木聚糖酶和内切葡聚糖酶，这些酶可通过凝胶过滤色谱法分离。主要的内切葡聚糖酶不与伴刀豆球蛋白A-琼脂糖结合，也不能用高碘酸/席夫试剂染色。它在聚丙烯酰胺等电聚焦（pI = 7.1）上是均一的，通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳测得其分子量为12500。该内切葡聚糖酶从纤维素产生葡萄糖和寡糖混合物；纯化后的酶具有较小的葡聚糖酶活性。它在50℃和pH 6条件下稳定。
