通过凝胶过滤法对疣孢漆斑菌纤维素酶进行分级分离。

SELBY K, MAITLAND C C

Biochem J. 1965 Mar;94(3):578-83. doi: 10.1042/bj0940578.

Culture filtrates from Myrothecium verrucaria have been fractionated by gel filtration on Sephadex G-75 to give three major cellulolytic components with molecular weights of about 55000, 30000 and 5300. 2. The middle component has the bulk (90%) of the total carboxymethylcellulase activity and is little affected by exposure to cotton. The other two, which are mainly responsible for the activity of the filtrate towards cotton, are removed or deactivated by exposure to it. These observations accord with the previously reported behaviour of the whole culture filtrate. There is no evidence for interconversion of, or synergism between, these components. 3. Temperature control during gel filtration is necessary for reproducible results at high resolution. The effect of a change in temperature has been explained in terms of changes in the degree of swelling of the gel particles.

疣孢漆斑菌的培养滤液通过在葡聚糖G - 75上进行凝胶过滤进行分级分离，得到三种主要的纤维素分解成分，分子量分别约为55000、30000和5300。2. 中间成分具有总羧甲基纤维素酶活性的大部分（90%），并且受接触棉花的影响很小。另外两种成分主要负责滤液对棉花的活性，接触棉花后会被去除或失活。这些观察结果与之前报道的整个培养滤液的行为一致。没有证据表明这些成分之间会相互转化或存在协同作用。3. 在凝胶过滤过程中控制温度对于获得高分辨率的可重复结果是必要的。温度变化的影响已根据凝胶颗粒膨胀程度的变化进行了解释。

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

SELBY K, MAITLAND C C

Biochem J. 1965 Mar;94(3):578-83. doi: 10.1042/bj0940578.

Culture filtrates from Myrothecium verrucaria have been fractionated by gel filtration on Sephadex G-75 to give three major cellulolytic components with molecular weights of about 55000, 30000 and 5300. 2. The middle component has the bulk (90%) of the total carboxymethylcellulase activity and is little affected by exposure to cotton. The other two, which are mainly responsible for the activity of the filtrate towards cotton, are removed or deactivated by exposure to it. These observations accord with the previously reported behaviour of the whole culture filtrate. There is no evidence for interconversion of, or synergism between, these components. 3. Temperature control during gel filtration is necessary for reproducible results at high resolution. The effect of a change in temperature has been explained in terms of changes in the degree of swelling of the gel particles.

疣孢漆斑菌的培养滤液通过在葡聚糖G - 75上进行凝胶过滤进行分级分离，得到三种主要的纤维素分解成分，分子量分别约为55000、30000和5300。2. 中间成分具有总羧甲基纤维素酶活性的大部分（90%），并且受接触棉花的影响很小。另外两种成分主要负责滤液对棉花的活性，接触棉花后会被去除或失活。这些观察结果与之前报道的整个培养滤液的行为一致。没有证据表明这些成分之间会相互转化或存在协同作用。3. 在凝胶过滤过程中控制温度对于获得高分辨率的可重复结果是必要的。温度变化的影响已根据凝胶颗粒膨胀程度的变化进行了解释。