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激光作为真菌学工具。

Lasers as tools for mycology.

作者信息

Thibaut M, Ansel M, de Azevedo Carneiro J

出版信息

Br J Exp Pathol. 1983 Aug;64(4):403-10.

PMID:6351892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2040789/
Abstract

Methods of preparing fungal samples for chemical analysis are briefly considered. The basic principles employed in laser microscopy are outlined. The applications of the technique to mycology and to plant biology are discussed. Such a method allows chemical information to be obtained on the same sample and permits the detection of 74 elements in the periodic system of Mendeleev from lithium (3) to uranium (92). The preparative procedures are evaluated according to general histochemical principles. This technique requires little specimen preparation and has the advantage of not requiring large masses of tissue.

摘要

简要介绍了制备用于化学分析的真菌样本的方法。概述了激光显微镜所采用的基本原理。讨论了该技术在真菌学和植物生物学中的应用。这种方法能够在同一样本上获取化学信息,并能检测门捷列夫周期表中从锂(3)到铀(92)的74种元素。根据一般组织化学原理对制备程序进行了评估。该技术所需的样本制备很少,且具有无需大量组织的优点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/631b3ef836b0/brjexppathol00100-0065-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/81360a5ed0da/brjexppathol00100-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/cde4be921800/brjexppathol00100-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/631b3ef836b0/brjexppathol00100-0065-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/81360a5ed0da/brjexppathol00100-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/cde4be921800/brjexppathol00100-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cabb/2040789/631b3ef836b0/brjexppathol00100-0065-b.jpg

相似文献

1
Lasers as tools for mycology.激光作为真菌学工具。
Br J Exp Pathol. 1983 Aug;64(4):403-10.
2
Study of allergenic fungi by means of a laser beam.利用激光束对致敏真菌的研究。
Br J Exp Pathol. 1979 Apr;60(2):180-4.
3
A microanalysis approach to investigate problems encountered in mycology.一种用于研究真菌学中遇到问题的微观分析方法。
Am J Pathol. 1978 Jan;90(1):23-32.
4
Observations on the chemical composition of Trichothecium roseum link determined by X-ray microanalysis and by laser microscopy.
Microbios. 1983;38(153-154):139-50.
5
Application of the laser to the study of pathogenic fungi.
Med Microbiol Immunol. 1979 May 15;167(2):99-105. doi: 10.1007/BF02123559.
6
[New compound--polymeric iron orthophosphate--in various fungi].
Dokl Akad Nauk SSSR. 1971 Aug 11;199(5):1204-5.
7
Modern physical methods for analysing elements and structures in histochemistry.组织化学中用于分析元素和结构的现代物理方法。
Acta Histochem. 1986;80(2):205-13. doi: 10.1016/S0065-1281(86)80065-4.
8
[Electron optical demonstration of acid mucopolysaccharides in fungal hyphae].
Naturwissenschaften. 1967 Oct;54(19):521. doi: 10.1007/BF01129383.
9
The lipids of thermophilic fungi: lipid composition comparisons between thermophilic and mesophilic fungi.嗜热真菌的脂质:嗜热真菌与嗜温真菌脂质组成的比较
Lipids. 1970 Jan;5(1):100-3. doi: 10.1007/BF02531102.
10
X-ray microanalysis as applied to fungal tissues.应用于真菌组织的X射线微分析。
Br J Exp Pathol. 1977 Apr;58(2):209-14.

本文引用的文献

1
Laser activation of rapid absorption changes in spinach chloroplasts and chlorella.菠菜叶绿体和小球藻中激光激活的快速吸收变化
Plant Physiol. 1966 Jun;41(6):983-91. doi: 10.1104/pp.41.6.983.
2
MICROSURGERY OF LIVING CELLS BY RUBY LASER IRRADIATION.红宝石激光辐照下活细胞的显微外科手术。
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3
SPECTROSCOPIC ULTRAMICROANALYSIS WITH A LASER.
Science. 1963 Oct 11;142(3589):236-7. doi: 10.1126/science.142.3589.236.
4
Ruby laser as a microsurgical instrument.
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5
Experimental Candida albicans chorioretinitis treated by laser.
Am J Ophthalmol. 1967 Mar;63(3):440-6. doi: 10.1016/0002-9394(67)90241-3.
6
The laser microprobe: its use for elemental analysis in histochemistry.
J Histochem Cytochem. 1966 Nov;14(11):862-8. doi: 10.1177/14.11.862.
7
Laser microscope irradiation of Physarum polycephalum: dynamic and ultrastructural effects.多头绒泡菌的激光显微镜照射:动态和超微结构效应
J Cell Biol. 1969 Jan;40(1):108-19. doi: 10.1083/jcb.40.1.108.
8
Microdissection of developmental stages of the cellular slime mold, Dictyostelium discoideum, using a ruby laser.
Dev Biol. 1974 Jul;39(1):183-8. doi: 10.1016/s0012-1606(74)80022-9.
9
Photocoagulation of active and inactive lesions of presumed ocular histoplasmosis.
Am J Ophthalmol. 1975 Mar;79(3):428-36. doi: 10.1016/0002-9394(75)90617-0.